Human T-Cell leukemia virus type 1 Tax protein transforms rat fibroblasts via two distinct pathways
Human T-Cell leukemia virus type 1 Tax protein transforms rat fibroblasts via two distinct pathways
复制标题
DOI：
10.1128/jvi.71.6.4445-4451.1997
复制
发表时间：
1997-06-01
影响因子：
5.4
通讯作者：
Fujii, M
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Shibata, H;Fujii, M
人类T细胞白血病病毒1型（HTLV-1）Tax蛋白激活几种细胞基因的转录。该功能被认为在HTLV-1白血病发生的Tax依赖性转化步骤中起关键作用。Tax通过三个增强子激活转录：环AMP反应元件（CRE）样序列、κ B元件和CArG盒。通过软琼脂中Rat-1细胞的集落形成和大鼠胚胎成纤维细胞（REF）的Ras协同焦点形成来检测它们参与Tax转化大鼠成纤维细胞。在Tax突变体中，那些保留CArG盒活性的突变体像野生型Tax一样转化REF，而那些对CArG盒无活性的突变体则没有。因此，CArG盒途径的激活对于Tax转化REF至关重要。相反，KB元件的激活与Tax对Rat-1的转化相关。这些结果表明Tax通过两种不同的途径转化大鼠成纤维细胞。
The human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates the transcription of several cellular genes. This function is thought to play a critical role in the Tax-dependent transformation step in HTLV-1 Ieukemogenesis. Tax activates transcription via three enhancers: the cyclic AMP response element (CRE)-like sequence, the kappa B element, and the CArG box. Their involvement in the transformation of rat fibroblasts by Tax aas examined by colony formation of Rat-1 cells in soft agar and Ras cooperative focus formation of rat embryo fibroblasts (REF). Among Tax mutants, those retaining activity for the CArG box transformed REF like wild-type Tax, while those inactive for the CArG box did not. Thus, the activation of the CArG box pathway is essential for the transformation of REF by Tax. In contrast, activation of the KB element correlated with the transformation of Rat-1 by Tax. These results show that Tax transforms rat fibroblasts via two distinct pathways.