Plasmid content of Streptococcus faecalis strain 39-5 and identification of a pheromone (cPD1)-induced surface antigen.
Plasmid content of Streptococcus faecalis strain 39-5 and identification of a pheromone (cPD1)-induced surface antigen.
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粪链球菌菌株 39-5 的质粒含量和信息素 (cPD1) 诱导的表面抗原的鉴定。
DOI：
10.1099/00221287-129-4-1207
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Clewell,DB
中科院分区：
文献类型：
--
作者：
Yagi,Y;Kessler,RE;Shaw,JH;Lopatin,DE;An,F;Clewell,DB
粪链球菌 39-5 是一种溶血性、致细菌性菌株，含有 6 个质粒。其中一个质粒 pPD1 (36·4 MDal) 确定细菌素并编码对受体（无质粒）菌株分泌的性信息素 cPD1 的接合反应。信息素反应的特点是形成供体（反应体）与受体的交配聚集体。聚集需要磷酸盐和二价阳离子的存在，并且会受到破坏蛋白质结构的试剂或条件的抑制。推测聚集是由于供体细胞表面上新的蛋白质分子的合成所致。这种材料被称为“聚集物质”，经鉴定并发现其表现出与未诱导细胞无关的抗原特性；可以通过免疫电子显微镜检测到。交叉免疫电泳证明，可以从诱导细胞中提取聚集物质，但不能从未诱导细胞中提取聚集物质。针对 pPD1 控制的聚集物质产生的抗体与其他质粒系统确定的聚集物质发生交叉反应，这些质粒系统对与 cPD1 无关的信息素做出反应。
Streptococcus faecalis39–5 is a haemolytic, bacteriocinogenic strain harbouring six plasmids. One of these plasmids, pPD1 (36·4 MDal) determines a bacteriocin and encodes a conjugative response to the sex pheromone cPD1 excreted by recipient (plasmid-free) strains. The pheromone response is characterized by the formation of mating aggregates of donors (responders) with recipients. Aggregation required the presence of phosphate and divalent cations and was inhibited by agents or conditions that destroy protein structure. Aggregation was postulated to be due to synthesis of a new proteinaceous molecule on the donor cell surface. Referred to as ‘aggregation substance’, such a material was identified and found to exhibit antigenic properties not associated with uninduced cells; it could be detected by immunoelec-tron microscopy. Aggregation substance could be extracted from induced cells but not uninduced cells as demonstrated by crossed immunoelectrophoresis. Antibody raised against the aggregation substance controlled by pPD1 cross-reacted with aggregation substance determined by other plasmid systems which respond to pheromones unrelated to cPD1.