MORPHINE ACTIVATION OF C-FOS EXPRESSION IN RAT-BRAIN
MORPHINE ACTIVATION OF C-FOS EXPRESSION IN RAT-BRAIN
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DOI：
10.1016/s0006-291x(88)80306-1
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发表时间：
1988-12-15
影响因子：
3.1
通讯作者：
HARLAN, RE
中科院分区：
文献类型：
--
作者：
CHANG, SL;SQUINTO, SP;HARLAN, RE
通过观察吗啡对大鼠尾壳核中富含的原癌基因c-fos及其编码核蛋白pp55c-fos （FOS）的激活作用，探讨了阿片作用的受体后机制。型阿片受体。急性吗啡治疗后，大鼠尾壳核c-fos mRNA水平在45分钟时达到最大值（为对照水平的420%），90分钟时恢复到对照水平。吗啡拮抗剂纳洛酮完全消除了这种诱导作用。免疫细胞化学检测的Fos蛋白在吗啡注射后3小时在尾壳核中也有升高，但在嗅结节中没有，嗅结节没有。型阿片受体。吗啡激活阿片受体后，c-fos基因被激活，Fos蛋白可能作为信号换能器在基因调控水平上参与阿片成瘾机制。
The post-receptor mechanism of opiate action has been studied by examining the activation by morphine of the proto-oncogene c-fos and its encoded nucleoprotein pp55c-fos (FOS) in rat caudate-putamen, which is rich in the .mu.-type opiate receptor. Following an acute morphine treatment, c-fos mRNA levels in rat caudate-putamen were increased to maximum (420% of control level) at 45 minutes and returned to control levels at 90 minutes. This induction was completely abolished by naloxone, a morphine antagonist. Fos protein, detected by immunocytochemistry, was also increased 3 hours after morphine injection, in the caudate-putamen, but not in the olfactory tubercle, which does not have the .mu.-type opiate receptor. Upon activation of opiate receptors by morphine, the c-fos gene is activated and Fos protein may act as a signal transducer uniquely involved in the mechanism of opiate addiction at the level of gene regulation.