Experimental and imaging techniques for examining fibrin clot structures in normal and diseased states.
Experimental and imaging techniques for examining fibrin clot structures in normal and diseased states.
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DOI：
10.3791/52019
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发表时间：
2015-04-01
期刊：
影响因子：
--
通讯作者：
Averett RD
中科院分区：
文献类型：
--
作者：
Fan NK;Keegan PM;Platt MO;Averett RD
纤维蛋白是一种细胞外基质蛋白，负责维持血栓的结构完整性。在过去的几年里，人们对纤维蛋白进行了大量研究，包括合成、结构功能和凝块溶解的研究。然而，对于疾病患者产生的血栓的形态和结构特征仍然知之甚少。在这项研究中，提出的实验技术可以检查由于糖尿病和镰状细胞性贫血等疾病状态引起的异常凝块结构的形态差异。我们的研究重点是纤维蛋白凝块的制备和评估，以便使用各种实验测定和共聚焦显微镜评估形态差异。此外，还描述了一种允许连续、实时计算纤维蛋白凝块溶解率的方法。本文描述的技术对于寻求阐明糖尿病或镰状细胞病患者的共病血栓形成病理学（例如心肌梗塞、缺血性心脏病和中风）的研究人员和临床医生来说非常重要。
Fibrin is an extracellular matrix protein that is responsible for maintaining the structural integrity of blood clots. Much research has been done on fibrin in the past years to include the investigation of synthesis, structure-function, and lysis of clots. However, there is still much unknown about the morphological and structural features of clots that ensue from patients with disease. In this research study, experimental techniques are presented that allow for the examination of morphological differences of abnormal clot structures due to diseased states such as diabetes and sickle cell anemia. Our study focuses on the preparation and evaluation of fibrin clots in order to assess morphological differences using various experimental assays and confocal microscopy. In addition, a method is also described that allows for continuous, real-time calculation of lysis rates in fibrin clots. The techniques described herein are important for researchers and clinicians seeking to elucidate comorbid thrombotic pathologies such as myocardial infarctions, ischemic heart disease, and strokes in patients with diabetes or sickle cell disease.
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影响因子：
2.9
作者：
Keegan PM;Surapaneni S;Platt MO
通讯作者：
Platt MO
DOI：
10.1161/01.atv.0000241589.52950.4c
发表时间：
2006-11-01
影响因子：
8.7
作者：
Collet, J. P.;Allali, Y.;Montalescot, G.
通讯作者：
Montalescot, G.
影响因子：
8.2
作者：
Dunn, EJ;Philippou, H;Grant, PJ
通讯作者：
Grant, PJ
影响因子：
8.2
作者：
MAYNE, EE;BRIDGES, JM;WEAVER, JA
通讯作者：
WEAVER, JA
影响因子：
8.7
作者：
Collet, JP;Park, D;Weisel, JW
通讯作者：
Weisel, JW