Characterization of isolated nitrogenase FeVco.
Characterization of isolated nitrogenase FeVco.
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DOI：
10.1021/ja1019657
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发表时间：
2010-09-15
影响因子：
15
通讯作者：
Ribbe, Markus W.
中科院分区：
文献类型：
--
作者：
Fay, Aaron W.;Blank, Michael A.;Lee, Chi Chung;Hu, Yilin;Hodgson, Keith O.;Hedman, Britt;Ribbe, Markus W.
Mo-和V-固氮酶的辅因子（即，FeMoCo和FeVCo）是具有不同催化性质的同源金属中心。迄今为止，仅报道了从褐褐固氮菌（Azotobacter chroocucum）中分离到FeVco。然而，这种分离的FeVco物质不具有完全的底物还原能力，因为它不能恢复辅因子缺陷的MoFe蛋白的N2还原能力。在这里，我们报告的分离和表征的一个完全活跃的物种FeVco棕色固氮菌。我们的金属和活性分析表明，FeVco已被完整提取，具有将C2 H2还原为C2 H6的特征能力，也许更重要的是，具有将N2还原为NH3的能力。此外，我们的EPR和XAS/EXAFS研究表明，FeVco是类似的，但不同的FeMoco的电子性质和结构拓扑结构，这可以解释的差异，在反应的两个辅因子。这项研究的结果不仅允许的第一个基于EXAFS的结构模型的建议，孤立FeVco，但也奠定了基础，为未来的催化和结构调查这一独特的类簇。
The cofactors of the Mo- and V-nitrogenases (i.e., FeMoco and FeVco) are homologous metal centers with distinct catalytic properties. So far, there has been only one report on the isolation of FeVco from Azotobacter chroocucum. However, this isolated FeVco species did not carry the full substrate-reducing capacity, as it is unable to restore the N2-reducing ability of the cofactor-deficient MoFe protein. Here, we report the isolation and characterization of a fully active species of FeVco from Azotobacter vinelandii. Our metal and activity analyses show that FeVco has been extracted intact, carrying with it the characteristic capacity to reduce C2H2 to C2H6 and, perhaps even more importantly, the ability to reduce N2 to NH3. Moreover, our EPR and XAS/EXAFS investigations indicate that FeVco is similar to, yet distinct from FeMoco in electronic properties and structural topology, which could account for the differences in the reactivity of the two cofactors. The outcome of this study not only permits the proposal of the first EXAFS-based structural model of the isolated FeVco, but also lays a foundation for future catalytic and structural investigations of this unique metallocluster.
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影响因子：
56.9
作者：
Einsle, O;Tezcan, FA;Rees, DC
通讯作者：
Rees, DC
影响因子：
4.6
作者：
RANDALL, CR;SHU, LJ;QUE, L
通讯作者：
QUE, L
影响因子：
4.4
作者：
CORBIN, JL
通讯作者：
CORBIN, JL
影响因子：
15
作者：
Sarangi, Ritimukta;Aboelella, Nermeen;Solomon, Edward I.
通讯作者：
Solomon, Edward I.
DOI：
10.1073/pnas.0904408106
发表时间：
2009-06-09
影响因子：
11.1
作者：
Lee, Chi Chung;Hu, Yilin;Ribbe, Markus W.
通讯作者：
Ribbe, Markus W.