In utero exposure to low-dose 2,3′,4,4′,5-pentachlorobiphenyl (PCB 118) impairs male fertility and alters neurobehavior in rat offspring
In utero exposure to low-dose 2,3′,4,4′,5-pentachlorobiphenyl (PCB 118) impairs male fertility and alters neurobehavior in rat offspring
复制标题
DOI：
10.1016/j.tox.2004.05.006
复制
发表时间：
2004-10-01
期刊：
影响因子：
4.5
通讯作者：
Chahoud, I
中科院分区：
文献类型：
--
作者：
Kuriyama, SN;Chahoud, I
在子宫内暴露于与人类暴露水平相当的低剂量PCB 118后，对大鼠后代的神经行为（运动活动和发育反射）和雄性生殖参数进行了评估。Sprague-Dawley母鼠在妊娠第6天通过灌胃给予单剂量375 μ g PCB 118/kg体重或花生油（对照）。经计算，该剂量比人类母乳中的剂量高约100倍。在暴露于PCB 118后，对大鼠后代的出生后反射、运动活动和雄性生殖能力进行了评估。对青春期（PND 70-74）连续5天的自发活动进行评价，发现接触PCB 118的母鼠的后代活动过度。在成年雄性（PND 170）中，在睾丸、附睾和精囊（绝对和相对重量）较小的接触多氯联苯的动物中观察到对生殖器官的明显影响。还观察到精子和精子细胞数量减少以及每日精子产量受损。我们的研究结果清楚地表明，低剂量暴露于PCB 118会改变神经行为，并损害后代的成年男性生育能力。这与报告的大鼠在接触高剂量多氯联苯后精子产量和睾丸重量增加形成对比。多氯联苯似乎具有可变的剂量相关效应，因此，低剂量研究对于全面了解人类风险评估非常重要。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Neurobehavior (motor activity and developmental reflexes) and male reproductive parameters were evaluated in rat offspring after in utero exposure to a low dose of PCB 118 comparable to human exposure levels. Sprague-Dawley dams were treated on gestation day 6 by gavage with a single dose of 375 mug PCB 118/kg body weight or peanut oil (control). The dose was calculated to be approximately 100-fold higher than that found in human breast milk. Postnatal reflexes, motor activity and male reproductive performance were evaluated in rat offspring after exposure to PCB 118. Evaluation of locomotor activity for five consecutive days during puberty (PND 70-74) revealed hyperactivity in offspring from PCB 118-exposed dams. In adult males (PND 170), clear effects on reproductive organs were observed in PCB-exposed animals which had smaller testes, epididymides, and seminal vesicles (absolute and relative weights). Decreases in sperm and spermatid numbers and impairment of daily sperm production were also observed. Our results clearly demonstrate that low-dose exposure to PCB 118 alters neurobehavior and impairs adult male fertility in offspring. This is in contrast to the reported increases in sperm production and testis weight in rat after high dose PCB exposures. PCBs appear to possess variable dose-related effects and therefore low-dose studies are important to obtain a complete picture for human risk assessment. (C) 2004 Elsevier Ireland Ltd. All rights reserved.