Induction of aberrant agonistic behavior by a combination of serotonergic and dopaminergic manipulation in rats
Induction of aberrant agonistic behavior by a combination of serotonergic and dopaminergic manipulation in rats
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DOI：
10.1007/s00429-021-02238-3
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发表时间：
2021-02
影响因子：
3.1
通讯作者：
N. Kai;S. Ueda
中科院分区：
文献类型：
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作者：
N. Kai;S. Ueda
5-羟色胺（5-HT）和多巴胺（DA）参与社会行为的调节。然而，它们的相互作用对社会行为的影响还没有得到很好的理解。在本研究中，大鼠接受了中缝核内注射多巴胺能神经毒素和/或全身给予l-3，4-二羟基苯丙氨酸（l-DOPA），并使用居民-入侵者（RI）范式研究了它们的激动行为。在DA + /5-HT-组，这两个单胺能治疗，表现出强烈的跳跃和飞行反应的入侵者。这些行为在只接受5-HT损伤或l-DOPA治疗的大鼠中没有观察到。为了解决这些异常行为的神经基础，我们比较了不同组之间的c-Fos免疫反应性。DA + /5-HT-组在下丘脑腹内侧核、乳头体前核和导水管周围灰质等与反捕食防御行为相关的区域有c-Fos激活。此外，这组增加c-Fos表达在腹后部的前嗅核（AOVP）。为了测试该区域在异常行为中的参与，在单胺能处理之前在AOVP中进行细胞毒性损伤，并使用RI试验检查随后的行为。AOVP损毁DA + /5-HT-大鼠的异常行为减轻。这些结果表明，AOVP参与了异常防御行为的产生，5-HT/DA平衡在社会行为的调节中是重要的。
Serotonin (5-HT) and dopamine (DA) are involved in the regulation of social behaviors. However, the effects of their interactions on social behavior are not well understood. In this study, rats received a serotonergic neurotoxin injection into the raphe nuclei and/or systemic administration ofl-3, 4-dihydroxyphenylalanine (l-DOPA), and their agonistic behaviors were investigated using the resident–intruder (RI) paradigm. Rats in the DA + /5-HT-group, which were administered both monoaminergic treatments, exhibited intense jump and flight responses to intruders. These behaviors were not observed in rats that received either 5-HT lesions orl-DOPA treatment only. To address the neural basis of these aberrant behaviors, we compared c-Fos immunoreactivity in the brain among the different groups. The DA + /5-HT-group had c-Fos activation in areas related to anti-predatory defensive behaviors, such as the ventromedial hypothalamic nucleus, premammillary nucleus, and periaqueductal gray. Moreover, this group had increased c-Fos expression in the ventroposterior part of the anterior olfactory nucleus (AOVP). To test the involvement of this area in the aberrant behaviors, cytotoxic lesions were performed in the AOVP prior to the monoaminergic treatments, and subsequent behaviors were examined using the RI test. The AOVP-lesioned DA + /5-HT-rats had attenuation of the aberrant behaviors. Together, these results suggest that the AOVP is involved in the generation of the aberrant defensive behaviors, and that 5-HT/DA balance is important in the regulation of social behaviors.