Neointima formation in the rat carotid artery is exacerbated by dietary copper deficiency
Neointima formation in the rat carotid artery is exacerbated by dietary copper deficiency
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DOI：
10.1177/153537020222700713
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发表时间：
2002-07-01
影响因子：
3.2
通讯作者：
Schuschke, DA
中科院分区：
文献类型：
--
作者：
Lucca, JJD;Saari, JT;Schuschke, DA
膳食铜是一种必需的微量元素，在心血管系统的功能和结构方面发挥作用。特别是，已知缺铜大鼠的血管对炎症刺激的反应显着增强。目前的研究旨在量化喂养充足或缺乏铜的饮食的大鼠中损伤引起的新内膜增殖和狭窄的程度。给雄性断奶的 SpragueDawley 大鼠喂食铜含量充足（CuA；5.6 杯 Cu/g）或缺乏铜（CuD；0.3 杯 Cu/g）的纯化饮食，持续 4 周。在左颈外动脉中诱发球囊损伤。损伤后十四天，颈动脉横截面的组织形态学分析显示，与 CuA 对照组相比，CuD 组的新内膜形成增加（新内膜/中膜比率：分别为 4.55 +/- 0.93 vs 1.45 +/- 0.2）。这些结果与表明喂食这种 CuD 饮食的大鼠的铜/锌超氧化物歧化酶 (SOD) 活性降低的数据相对应。由于已知超氧阴离子和氧化还原状态在响应损伤的新内膜形成程度中发挥关键作用，因此我们认为 CuD 组中观察到的过度新内膜增殖是 Cu/Zn-SOD 活性减弱的结果。
Dietary copper is an essential trace element with roles in both functional and structural aspects of the cardiovascular system. In particular, the vascular response to inflammatory stimuli is known to be significantly augmented in copper-deficient rats. The current study was designed to quantify the extent of injury-induced neointimal proliferation and stenosis in rats fed diets either adequate or deficient in copper. Male, weanling SpragueDawley rats were fed purified diets that were either adequate (CuA; 5.6 mug Cu/g) or deficient (CuD; 0.3 mug Cu/g) in copper for 4 weeks. Balloon injury was induced in the left external carotid arteries. Fourteen days after injury, histomorphometric analysis of cross-sections from carotid arteries showed increased neo-intimal formation in the CuD group compared with the CuA controls (neointima/media ratio: 4.55 +/- 0.93 vs 1.45 +/- 0.2, respectively). These results correspond with data indicating that the activity of Cu/Zn-superoxide dismutase (SOD) is depressed in rats fed this CuD diet. Because superoxide anion and redox status are known to play a key role in the extent of neointimal formation in response to injury, we propose that the exaggerated neointimal proliferation seen in the CuD group is the result of the diminished Cu/Zn-SOD activity.