NG2 proteoglycan and the actin-binding protein fascin define separate populations of actin-containing filopodia and lamellipodia during cell spreading and migration.
NG2 proteoglycan and the actin-binding protein fascin define separate populations of actin-containing filopodia and lamellipodia during cell spreading and migration.
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NG2 蛋白聚糖和肌动蛋白结合蛋白肌成束蛋白在细胞扩散和迁移过程中定义了含有肌动蛋白的丝状伪足和片状伪足的不同群体。
DOI：
10.1091/mbc.7.12.1977
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发表时间：
1996
影响因子：
3.3
通讯作者：
Stallcup,WB
中科院分区：
文献类型：
--
作者：
Lin,XH;Grako,KA;Burg,MA;Stallcup,WB
跨膜蛋白多糖NG2既能与细胞外基质成分相互作用，又能与肌动蛋白细胞骨架相互作用。对NG2在细胞扩散过程中的分布的研究表明，NG2可以与两种不同类型的含有肌动蛋白的细胞骨架结构联系在一起，这取决于来自底物的刺激的性质。在纤维连接蛋白包被的培养皿中，细胞表面NG2仅与细胞内形成的应力纤维相联系。在涂有多聚L赖氨酸的培养皿中，细胞表面NG2与从细胞外围延伸的放射状突起有关。在纤维连接蛋白/多L-赖氨酸混合物以及层粘连蛋白、肌腱蛋白和VI型胶原等基质成分上传播，产生具有马赛克特征的细胞，即NG2与这两种结构都相关。NG2阳性的放射状突起有别于含有束素的第二类放射状结构。NG2阳性的延伸似乎是单独的独立单位(丝足)，而筋蛋白与膜内的肌动蛋白肋骨有关(片状脂膜)。NG2阳性结构和束蛋白阳性结构通常位于展开细胞的相反两极，提示这两类细胞延伸在形态发生或迁移过程中可能在细胞极性的建立中发挥作用。时间推移成像证实在迁移细胞的前沿存在片状脂肪，而在后缘存在大量的丝状伪足。
The transmembrane proteoglycan NG2 is able to interact both with components of the extracellular matrix and with the actin cytoskeleton. An examination of the distribution of NG2 during cell spreading suggests that NG2 can associate with two distinct types of actin-containing cytoskeletal structures, depending on the nature of the stimulus derived from the substratum. On fibronectin-coated dishes, cell surface NG2 associates exclusively with stress fibers developing within the cell. On poly-L-lysine-coated dishes, cell surface NG2 is associated with radial processes extending from the cell periphery. Spreading on fibronectin/poly-L-lysine mixtures, as well as on matrix components such as laminin, tenascin, and type VI collagen, produces cells with mosaic characteristics, i.e., NG2 is associated with both types of structures. NG2-positive radial processes are distinct from a second population of radial structures that contain fascin. NG2-positive extensions appear to be individual self-contained units (filopodia), whereas fascin is associated with actin ribs within sheets of membrane (lamellipodia). NG2- and fascin-positive structures are often localized to opposite poles of spreading cells, suggesting a possible role for the two classes of cellular extensions in the establishment of cell polarity during morphogenesis or migration. Time lapse imaging confirms the presence of lamellipodia on the leading edges of migrating cells, while numerous filopodia are present on trailing edges.
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NG2 蛋白多糖与肌动蛋白细胞骨架的相互作用
影响因子：
4
作者：
Xiao;K. Dahlin‐Huppe;W. Stallcup
通讯作者：
W. Stallcup
从基质到细胞骨架的信号传导：细胞表面蛋白聚糖在基质组装中的作用。
DOI：
--
发表时间：
1996
期刊：
Kidney international. Supplement.
影响因子：
--
作者：
Woods,A;Couchman,JR
通讯作者：
Couchman,JR
从人胎盘中获得的独特胶原片段（内膜胶原）的结构多样性和结构域组成。
影响因子：
4.1
作者：
E. Odermatt;J. Risteli;Vera Van DELDENt;Rupert Timplt
通讯作者：
Rupert Timplt
DOI：
10.1016/s0021-9258(18)46961-x
发表时间：
1994-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jonathan Q. Davis;V. Bennett
通讯作者：
Jonathan Q. Davis;V. Bennett
影响因子：
--
作者：
MITCHISON, TJ
通讯作者：
MITCHISON, TJ