P-selectin mediates neutrophil adhesion to endothelial cell borders
P-selectin mediates neutrophil adhesion to endothelial cell borders
复制标题
DOI：
10.1002/jlb.65.3.299
复制
发表时间：
1999-03-01
影响因子：
5.5
通讯作者：
Smith, CW
中科院分区：
文献类型：
--
作者：
Burns, AR;Bowden, RA;Smith, CW
在急性炎症反应期间，内皮P-选择素（CD 62 P）可以介导从自由流动的血流中初始捕获中性粒细胞。P-选择素储存在分泌颗粒（韦伯-帕拉德小体）中，并且在组胺或凝血酶刺激后在内皮表面上快速表达。由于中性粒细胞迁移优先发生在内皮细胞边缘，我们希望确定是否P-选择素依赖性中性粒细胞捕获（粘附）发生在内皮细胞边缘。在静态或流体动力学流速（2 dyn/cm（2））条件下，组胺（10（-4）M）或凝血酶（0.2 U/mL）处理诱导中性粒细胞优先（大于或等于75%）粘附于内皮单层的细胞边缘。阻断抗体研究证实中性粒细胞粘附完全依赖于P-选择素。组胺处理后P-选择素表面表达显著增加，P-选择素免疫染色集中于沿着内皮边界。我们的结论是，优先P-选择素表达沿着内皮herders可能是一个重要的机制，针对中性粒细胞迁移内皮边界。
During an acute inflammatory response, endothelial P-selectin (CD62P) can mediate the initial capture of neutrophils from the free flowing bloodstream. P-selectin is stored in secretory granules (Weibel-Palade bodies) amid is rapidly expressed on the endothelial surface after stimulation with histamine or thrombin. Because neutrophil transmigration occurs preferentially at endothelial borders, we wished to determine whether P-selectin-dependent neutrophil capture (adhesion) occurs at endothelial cell borders. Under static or hydrodynamic now (2 dyn/cm(2)) conditions,, histamine (10(-4) M) or thrombin (0.2 U/mL) treatment induced preferential (greater than or equal to 75%) neutrophil adhesion to the cell borders of endothelial monolayers, Blocking antibody studies established that neutrophil adhesion was completely P-selectin dependent. P-selectin surface expression increased significantly after histamine treatment and P-selectin immunostaining was concentrated along endothelial borders. We conclude that preferential P-selectin expression along endothelial herders may be an important mechanism for targeting neutrophil migration at endothelial borders.