The murine gene, Traube, is essential for the growth of preimplantation embryos
The murine gene, Traube, is essential for the growth of preimplantation embryos
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DOI：
10.1006/dbio.2000.9915
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发表时间：
2000-11-15
影响因子：
2.7
通讯作者：
Gruss, P
中科院分区：
文献类型：
--
作者：
Thomas, T;Voss, AK;Gruss, P
对胚胎植入前发育的遗传控制知之甚少。我们已经分离，表征，并突变以前未描述的小鼠基因，Traube（Trb），植入前发育所必需的。在大鼠、人类和酵母中发现了类似的蛋白质编码序列。TRB蛋白含有两个氨基末端酸性结构域，亮氨酸拉链，和三个推定的核定位信号。Trb基因在发育早期普遍表达水平较低，从E11.5开始仅限于肝脏和中枢神经系统。Myc标记的TRB蛋白定位于细胞核，并且在大部分细胞中定位于核仁。Trb突变体胚胎在E2.5的致密桑椹胚阶段停止发育。在E3.5，它们开始分解，一天后它们解体并死亡。观察到的缺陷是细胞自主的，因为突变细胞未能参与嵌合胚胎的形成。Trb突变体胚胎显示总细胞数减少50%。突变体胚胎表现出缺乏的核糖体，多聚核糖体和粗面内质网。这种核糖体的缺乏以及TRB定位于核糖体合成位点的核仁，表明TRB参与了核糖体的合成。(C)北京大学出版社.
Little is known about the genetic control of preimplantation development. We have isolated, characterized, and mutated a previously undescribed mouse gene, Traube (Trb), essential for preimplantation development. Similar protein coding sequences are found in rats, humans, and yeast. The TRB protein contained two amino-terminal acidic domains, a leucine zipper, and three putative nuclear localization signals. The Trb gene was expressed at low levels ubiquitously early in development and became restricted to the liver and the central nervous system from E11.5 onward. Myc-tagged TRB protein was localized to the nucleus, and in a large proportion of the cells to the nucleoli. The Trb mutant embryos halted in development at the compacted morula stage at E2.5. At E3.5 they started to decompact and a day later they disintegrated and died. The observed defect was cell autonomous, as mutant cells failed to participate in the formation of chimeric embryos. The Trb mutant embryos showed a 50% reduction of the total cell number. The mutant embryos exhibited a paucity of ribosomes, polyribosomes, and rough endoplasmic reticulum. This paucity of ribosomes together with the localization of TRB to the nucleoli, the site of ribosome synthesis, suggests that TRB is involved in the synthesis of ribosomes. (C) 2000 Academic Press.