Amperometric biosensor coupled to capillary electrophoresis for glucose determination
Amperometric biosensor coupled to capillary electrophoresis for glucose determination
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DOI：
10.1007/s00604-005-0362-3
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发表时间：
2005-07-01
影响因子：
5.7
通讯作者：
Yang, PY
中科院分区：
文献类型：
--
作者：
Chen, G;Wang, YT;Yang, PY
一种新型葡萄糖生物传感器已被制造出来并用作毛细管电泳（CE）系统的电流检测器。通过浸涂法在直径500μm的圆盘铂电极上依次修饰(+/-)-1-二茂铁乙胺和壳聚糖。随后将修饰电极浸入葡萄糖氧化酶（GOx）溶液中，以将酶捕获在壳聚糖膜中。通过戊二醛交联1-二茂铁乙胺、GOx和壳聚糖的伯氨基，得到生物传感膜，从而减少1-二茂铁乙胺和GOx的浸出。研究了目标生物传感器的电化学行为。结果表明，所研究的生物传感器具有响应快、稳定性高、寿命长以及与CE系统理想的兼容性等特点。当采用 CE 将葡萄糖塞引入生物传感器表面时，电流响应在 0.0025 至 2.5 mM 范围内与葡萄糖浓度呈线性关系，在 +0.6 V 工作电位（相对于 SCE）下检测限为 1.2 μM (S/N = 3)。 CE-生物传感器系统用于测定人血清中的葡萄糖水平。结果令人满意，与医院化验结果非常吻合。
A novel glucose biosensor has been fabricated and employed as the amperometric detector of a capillary electrophoresis (CE) system. (+/-)-1-Ferrocenylethylamine and chitosan were successively modified on a 500-mu m diameter disc platinum electrode by dip-coating. The modified electrode was subsequently immersed in glucose oxidase (GOx) solution to entrap the enzyme in the chitosan membrane. The primary amino groups of 1-ferrocenylethylamine, GOx, and chitosan were cross-linked by glutaraldehyde to obtain a biosensing membrane so as to reduce leaching of 1-ferrocenylethylamine and GOx. The electrochemical behavior of the target biosensor was investigated. It was demonstrated that the investigated biosensor features fast response, high stability, long lifetime, and ideal compatibility with the CE system. When CE was employed to introduce a glucose plug into the surface of the biosensor, the current response was linear to the glucose concentration in the range of 0.0025 to 2.5 mM with a detection limit of 1.2 mu M (S/N = 3) at a working potential of +0.6 V (vs. SCE). The CE-biosensor system was applied to the determination of the glucose level in human serum. The results were satisfactory and in good agreement with the hospital assay results.