COMPARISON OF PROMOTER SUPPRESSION IN AVIAN AND MURINE RETROVIRUS VECTORS
COMPARISON OF PROMOTER SUPPRESSION IN AVIAN AND MURINE RETROVIRUS VECTORS
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DOI：
10.1093/nar/14.23.9381
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发表时间：
1986-12-09
影响因子：
14.9
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
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作者：
EMERMAN, M;TEMIN, HM
以前，我们描述了“启动子抑制”在感染性逆转录病毒载体与两个基因和一个内部启动子。在这里，我们研究了启动子抑制的几个参数，发现在一个整合的逆转录病毒载体的抑制量是依赖于载体是否来自脾坏死病毒或raurine白血病病毒和内部启动子是存在于载体。鼠白血病病毒载体比类似的脾坏死病毒载体表现出更少的抑制作用。此外，抑制的量不依赖于启动子的相对强度，也不依赖于启动子之间的距离。此外，我们发现，在其中一个启动子被抑制的载体中，启动子周围染色质的DNasel敏感性与其表达的抑制之间存在负相关性。
Previously, we described “promoter suppression” in infectious retrovirus vectors with two genes and an internal promoter. Here, we examined several parameters of promoter suppression and found that the amount of suppression in an integrated retrovirus vector was dependent both on whether the vector was derived from spleen necrosis virus or raurine leukemia virus and on which internal promoter was present in the vector. Hurine leukemia virus vectors showed less suppression than analogous spleen necrosis virus vectors. Furthermore, the amount of suppression was not dependent on either the relative strengths of the promoters nor the distance between the promoters. Moreover, we found that in vectors in which one promoter was suppressed, there was an inverse correlation between the DNasel sensitivity of the chromatin surrounding a promoter and the suppression of its expression.