Sheep as an important source of E-coli O157/O157:H7 in Turkey
Sheep as an important source of E-coli O157/O157:H7 in Turkey
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DOI：
10.1016/j.vetmic.2014.06.014
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发表时间：
2014-08-27
影响因子：
3.3
通讯作者：
Gencay, Yilmaz Emre
中科院分区：
文献类型：
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作者：
Gencay, Yilmaz Emre
大肠杆菌O 157：H7是一种重要的食源性致病菌，主要与牛有关。然而，越来越多的数据显示了绵羊作为E。coli O 157：H7载体。E.大肠杆菌O 157/O 157：H7的直肠肛门粘膜拭子和尸体海绵样本的100只羊带到屠宰场在Kirikkale进行了分析超过一年。分子特征（stx（1）、stx（2）、eaeA、hly、lpfA1-3、espA、eae-alpha 1、eae-alpha 2、eae-beta、eae-beta 1、eae-beta 2、eae-gamma 1、eae-gamma 2/theta、stx 1（c）、stx 1（d）、Stx 2（c）、stx 2（d）、stx 2（e）、stx 2（f）、stx 2（g）、bla（ampC）、泰特（A）、泰特（B）、泰特（C）、泰特（D）、泰特（E）、测定了79株分离菌的泰特（G）、sul 1、sul 2、floR、cmlA、strA、strB和aadA），并对20种抗生素进行了最低抑菌浓度测定。E. Colt O 157/O 157：H7在18%的绵羊中发现，在一岁羔羊中比羔羊和成熟绵羊更流行，雄性绵羊比雌性绵羊更流行，尽管没有类别（季节，性别或年龄范围）对流行率有显著影响。此外，志贺菌E. Colt（STEC）O 157：H7分别在2%和8%的绵羊粪便和尸体中检出。此外，在所有分离株中均检测到lpfA 1 -3和eae-γ 1。4株发酵山梨糖醇的大肠杆菌均未对所研究的抗生素产生耐药性。colt O 157分离株泰特（A）、sul 1和aadA均为阳性。这是土耳其第一项揭示绵羊尸体被潜在高致病性STEC O 157：H7菌株污染所带来的潜在公共卫生风险的研究。(C)2014爱思唯尔有限公司版权所有。
Escherichia coli O157:H7 is a globally important foodborne pathogen and has been mainly associated with cattle as the reservoir. However, accumulating data shows the importance of sheep as an E. coli O157:H7 vehicle. The presence of E. coli O157/O157:H7 in recto-anal mucosal swap and carcass sponge samples of 100 sheep brought to the slaughterhouse in Kirikkale were analyzed over a year. Molecular characteristics (stx(1), stx(2), eaeA, hly, lpfA1-3, espA, eae-alpha 1, eae-alpha 2, eae-beta, eae-beta 1, eae-beta 2, eae-gamma 1, eae-gamma 2/theta, stx1(c),stx1(d), Stx2(c), stx2(d), stx2(e), stx2(f), stx2(g), bla(ampC), tet(A), tet(B), tet(C), tet(D), tet(E), tet(G), sul1, sul2, floR, cmlA, strA, strB and aadA) of 79 isolates were determined and minimum inhibitory concentrations of 20 different antibiotics were investigated. E. colt O157/O157:H7 was found in 18% of sheep included in the study and was more prevalent in yearlings than lambs and mature sheep, and male than female sheep, though none of the categories (season, sex or age range) had significant effect on prevalence. Furthermore, Shiga-toxigenic E. colt (STEC) O157:H7 was determined in 2% and 8% of sheep feces and carcasses, respectively. Additionally, lpfA1-3 and eae-gamma 1 were detected in all isolates. None of the isolates showed resistance against investigated antibiotics, even though 4 sorbitol fermenting E. colt O157 isolates were positive for tet(A), sul1 and aadA. This is the first study in Turkey that reveals the potential public health risk due to the contamination of sheep carcasses with potentially highly pathogenic STEC O157:H7 strains. (C) 2014 Elsevier B.V. All rights reserved.