Preliminary antigenic characterization of an avian pneumovirus isolated from commercial turkeys in Colorado, USA
Preliminary antigenic characterization of an avian pneumovirus isolated from commercial turkeys in Colorado, USA
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DOI：
10.1080/03079459994407
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发表时间：
1999-12-01
期刊：
影响因子：
2.8
通讯作者：
Senne, DA
中科院分区：
文献类型：
--
作者：
Cook, JKA;Huggins, MB;Senne, DA
从美国科罗拉多州的火鸡中分离到一株禽肺炎病毒（Avian Pneumonovirus，APV），该病毒与早期的A、B亚群APV分离株具有相似的特征，命名为科罗拉多分离株（科罗拉多），当在鸡胚成纤维细胞（CEF）中传代7次或在CEF中传代7次后接着在火鸡中传代6次后使用时，在火鸡中诱导的临床症状与早期APV毒株引起的症状相似。尽管它在无特定病原体的鸡中诱导抗体应答，但未观察到临床体征。与亚组A或B不同，科罗拉多在气管器官培养物中未引起纤毛停滞，但在鸡胚成纤维细胞中产生了与其他APV毒株相同的典型细胞病变效应。A或B菌株的单特异性抗血清不能中和Colorado，反之亦然;中和亚群A或B菌株的单克隆抗体也不能中和科罗拉多。然而，它被A亚群超免疫血清部分中和。同源酶联免疫吸附试验（ELISA）抗原是检测科罗拉多抗体所必需的，因为使用A或B亚群菌株的ELISA检测科罗拉多抗体非常差。亚群A和B疫苗保护火鸡免受科罗拉多攻击。然而，虽然科罗拉多保护火鸡，并在一定程度上鸡，对亚组A株，对亚组B挑战的保护是不太好的两个species.These结果表明，科罗拉多应被归类为APV，但抗原差异表明，它不属于亚组A或B，并代表一个单独的亚组（亚组C）或可能是一个单独的血清型。
An avian pneumovirus (APV) isolated from turkeys showing respiratory disease in Colorado, USA, shared some characteristics with earlier subgroup A and B APV strains, This virus, designated the Colorado isolate (Colorado), when used after either seven passages in chick embryo fibroblasts (CEF), or seven passages in CEF followed by six turkey passages, induced clinical signs in turkeys that were similar to those caused by earlier APV strains. Although it induced an antibody response in specific pathogen free chickens, clinical signs were not seen. Unlike subgroups A or B, Colorado did not cause ciliostasis in tracheal organ cultures, but produced a cytopathic effect in chick embryo fibroblasts typical of that seen with other APV strains. Monospecific antisera to A or B strains did not neutralize Colorado and vice versa; nor did monoclonal antibodies, which neutralize subgroup A or B strains, neutralize Colorado. However, it was partially neutralized by a subgroup A hyperimmune serum, A homologous enzyme-linked immunosorbent assay (ELISA) antigen was essential for the detection of Colorado antibodies, since ELISAs in which subgroup A or B strains were used detected antibody to Colorado very poorly. Subgroup A and B vaccines protected turkeys against challenge with Colorado. However, while Colorado protected turkeys, and to some extent chickens, against subgroup A strains, protection against a subgroup B challenge was less good in both species.These results indicate that Colorado should be classified as an APV, but the antigenic differences suggest that it does not belong to subgroups A or B, and represents a separate subgroup (subgroup C) or possibly a separate serotype.