The Binding of Mercury by the Yeast Cell in Relation to Changes in Permeability
The Binding of Mercury by the Yeast Cell in Relation to Changes in Permeability
复制标题
酵母细胞对汞的结合与渗透性变化的关系
DOI：
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发表时间：
1960
期刊：
影响因子：
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通讯作者：
A. Rothstein
中科院分区：
文献类型：
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作者：
H. Passow;A. Rothstein
暴露于氯化汞的酵母细胞会对细胞膜造成不可逆的损害，导致钾和细胞阴离子流失到培养基中。K+的最大损失与汞浓度有关，但与K+损失的时间过程无关，在对数浓度与效应的图上，该关系遵循正态曲线。它的结论是，响应是所有或没有个别细胞，并且随着金属浓度的增加，超过阈值的细胞的比例增加。对汞与细胞结合的平行研究表明，有两个不同的阶段，其中只有一个阶段与生理反应有关。结合过程相对缓慢，但达到平衡状态。解吸作用明显地依赖于温度。汞的结合与生理反应（K+损失）之间不存在简单的化学计量关系。
Yeast cells exposed to mercuric chloride suffer irreversible damage to the membrane, resulting in a loss of potassium and cellular anions to the medium. The maximal loss of K+, but not the time course of K+ loss is related to the mercury concentration, the relationship following a normal curve on a graph of log-concentration versus effect. It is concluded that the response is all or none for individual cells, and that with increasing concentrations of metal, the threshold is exceeded in an increasing proportion of the cells. Parallel studies of the binding of mercury by the cells indicate two distinct phases, only one of which is associated with the physiological response. The binding process is relatively slow but reaches an equilibrium state. Desorption is markedly dependent on temperature. No simple stoichiometric relationship exists between the binding of mercury and the physiological response (K+ loss).