MiR-210-3p-EphrinA3-PI3K/AKT axis regulates the progression of oral cancer
MiR-210-3p-EphrinA3-PI3K/AKT axis regulates the progression of oral cancer
复制标题
MiR-210-3p-EphrinA3-PI3K/AKT 轴调节口腔癌的进展。
DOI：
10.1111/jcmm.15036
复制
发表时间：
2020-04-01
影响因子：
5.3
通讯作者：
Shang, Zhengjun
中科院分区：
文献类型：
--
作者：
Wang, Lin;Song, Yong;Shang, Zhengjun
本研究旨在探索新的治疗靶点，提高口腔鳞癌患者的生存率。检测miR-210- 3 p、EphrinA 3和EMT相关指标在口腔鳞癌组织和细胞系中的表达。此外，通过分子生物学技术、体外功能实验和肿瘤异种移植模型探索了EphrinA 3差异表达与肿瘤进展之间的关系。EphrinA 3（r（s）=-0.719，P < .05）和E-cadherin（r（s）=-0.856，P < .05）的表达与口腔鳞癌的病理分级呈负相关。蛋白质聚类显示EphrinA 3可能与肿瘤进展相关。EphrinA 3还可以调节口腔癌细胞的生物学行为。它通过PI 3 K/AKT信号通路调节EMT。miR-210- 3 p靶向基因EFNA 3。miR-210- 3 p表达上调可降低EphrinA 3的表达，进而影响OSCC的生物学行为。miR-210- 3 p-EphrinA 3-PI 3 K/AKT信号转导轴在口腔鳞癌的发生发展中起重要作用。EphrinA 3可作为口腔癌治疗的新靶点。
This study aimed to explore new therapeutic targets to improve the survival rate of patients with oral squamous cell carcinoma (OSCC). MiR-210-3p, EphrinA3 and EMT related indices were evaluated in OSCC tissues and cell lines. In addition, the relationship between differential EphrinA3 expression and tumour progression was explored through molecular biology techniques, in vitro functional experiments and tumour xenotransplantation models. The expression of EphrinA3 (r(s) = -0.719, P < .05) and E- cadherin (r(s) = -0.856, P < .05) was negatively correlated with the pathological grading in OSCC tissues. Protein clustering shows EphrinA3 may be associated with tumour progression. EphrinA3 also can regulate the biological behaviour of oral cancer cells. And it regulates the EMT by the PI3K/AKT signalling pathway. MiR-210-3p targeted the gen EFNA3. Up-regulation of miR-210-3p expression can decrease the expression of EphrinA3 and further to influence the biological behaviour of OSCC. The miR-210-3p-EphrinA3-PI3K/AKT signalling axis plays an important role in the progress of OSCC. EphrinA3 may serve as a novel target for oral cancer treatment.