Beneficial effects of Oridonin on myocardial ischemia/reperfusion injury: Insight gained by metabolomic approaches
Beneficial effects of Oridonin on myocardial ischemia/reperfusion injury: Insight gained by metabolomic approaches
复制标题
冬凌草甲素对心肌缺血/再灌注损伤的有益作用：通过代谢组学方法获得的见解
DOI：
10.1016/j.ejphar.2019.172587
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发表时间：
2019-10-15
影响因子：
5
通讯作者：
Xu, Xia
中科院分区：
文献类型：
--
作者：
Zhang, Junhong;Zhou, Yuanyuan;Xu, Xia
冬凌草甲素是从冬凌草Rabdosia rubescens（Hemsl.）原是中国著名的花草茶，具有多种健康益处。为了更好地了解冬凌草甲素的潜在心脏保护作用，我们采用超高效液相色谱-串联质谱（UPLC-MS/MS）代谢组学方法研究了冬凌草甲素预处理和未预处理心肌缺血/再灌注（MI/R）损伤后大鼠心脏组织和血清中的代谢变化。将大鼠随机分为对照组、假手术组、MI/R组和冬凌草甲素（10 mg/kg）+MI/R组。再灌注24 h后，收集心脏组织和血清进行生化和代谢组学分析。冬凌草甲素预处理可明显缩小心肌梗死面积，逆转血清心肌酶谱异常升高。此外，冬凌草甲素还参与了糖酵解、支链氨基酸、犬尿氨酸、精氨酸、谷氨酰胺和胆汁酸等代谢途径的调节。提示冬凌草甲素主要通过调节能量和氨基酸代谢发挥心肌保护作用。
Oridonin is a diterpenoid isolated from Rabdosia rubescens (Hemsl.) Hara, a well-known herbal tea in China with many health benefits. To provide a better understanding of the potential cardioprotective effect of Oridonin, we investigated the metabolic alterations in heart tissue and serum of rat subjected to myocardial ischemia/reperfusion (MI/R) injury with or without pretreatment of Oridonin by UPLC-MS/MS metabolomics approach. Rats were randomly divided into groups as follows: Control, Sham, MI/R and pretreated with Oridonin (10 mg/kg) + MI/R. After 24 h of reperfusion, heart tissue and serum were collected for biochemical and metabolomic analysis. Pretreatment with Oridonin significantly decreased infarct size and reversed the abnormal elevated myocardial zymogram in serum. Moreover, Oridonin regulated several metabolic pathways, including glycolysis, branched chain amino acid, kynurenine, arginine, glutamine and bile acid metabolism. Our results suggest that Oridonin indeed displays outstanding cardioprotective effect mainly by regulating energy and amino acid metabolism.