Screening and identification of Aspergillus activity against Xanthomonas oryzae pv. oryzae and analysis of antimicrobial components
Screening and identification of Aspergillus activity against Xanthomonas oryzae pv. oryzae and analysis of antimicrobial components
复制标题
曲霉菌抗米黄单胞菌活性的筛选和鉴定
DOI：
10.1007/s12275-019-8330-5
复制
发表时间：
2019-07-01
影响因子：
3
通讯作者：
Jiang, Donghua
中科院分区：
文献类型：
--
作者：
Jiang, Bei;Wang, Zhiying;Jiang, Donghua
筛选曲霉菌对米黄单胞菌 (Xanthomonas oryzae pv.) 的活性。米曲霉并分析所涉及的抗菌成分，60 曲霉菌属。从水果、土壤和其他栖息地中分离和纯化。 As-75，一种曲霉菌株，可以拮抗米黄单胞菌 pv。米曲霉是根据共培养期间形成的抑制区来鉴定的。根据形态学、ITS rDNA基因测序和系统发育树结果，该菌株与核盘曲霉表现出密切的同源性。生化表征测试表明，菌株As-75的发酵液表现出较高的环境适应能力。抗菌谱实验结果表明，As-75在体外对5种植物病原真菌和6种植物病原细菌表现出较强的拮抗活性。菌株 As-75 的发酵液在低于 60 摄氏度、pH 6.5 的荧光照射下表现出最大的稳定性。通过分级萃取、硅胶柱层析和薄层层析，从菌株As-75中得到具有拮抗活性的物质。通过质谱、核磁共振和电喷雾电离质谱(ESI-MS)分析，鉴定目标化合物为(2Z)-2-丁烯二酸-2-(1-甲基乙烯基)-4-甲酯；其分子量为170.06道尔顿，分子式为C8H10O4，是一种新型化合物。防治效果试验表明，化合物S1对水稻白叶枯病的防治效果优于对照。另外，M1处理方法效果较好，化合物S1对TN1、IR24、ZF802、中华11、武云粳21、日本晴6个水稻品种的白叶枯病防治效果分别为78.3%、77.5%、74.2%、75.3%、70.9%和72.1%。
To screen for Aspergillus activity against Xanthomonas oryzae pv. oryzae and analyse the antimicrobial components involved, 60 Aspergillus spp. were isolated and purified from fruits, soil and other habitats. As-75, an Aspergillus strain that can antagonize Xanthomonas oryzae pv. oryzae, was identified based on the zone of inhibition formed during co-culture. According to morphological, ITS rDNA gene sequencing and phylogenetic tree results, the strain showed close homology to Aspergillus sclerotiorum. The biochemical characterization tests showed that the fermentation broth of strain As-75 exhibited a high capacity for environmental adaptation. The results of the antimicrobial spectrum experiments demonstrated that As-75 exhibited fairly strong antagonistic activity against five plant pathogenic fungi and six plant pathogenic bacteria in vitro. The fermentation broth of strain As-75 displayed maximum stability under fluorescent illumination at temperatures below 60 degrees C at pH 6.5. A substance with antagonistic activity was obtained from strain As-75 via fractional extraction, silica gel column chromatography and thin-layer chromatography. Through mass spectrometry, nuclear magnetic resonance and electrospray ionization mass spectrometry (ESI-MS) analyses, the target compound was identified as (2Z)-2-butenedioic acid-2-(1-methylethenyl)-4-methyl ester; its molecular weight of 170.06 daltons and formula of C8H10O4 identify it as a novel compound. Trials of the preventative and curative effects demonstrated that compound S1 exhibited a better control efficiency than the control against rice bacterial blight. Additionally, the M1 processing method was better, and the efficiency of compound S1 in preventing rice bacterial blight in six rice varieties, TN1, IR24, ZF802, Zhonghua 11, Wuyunjing 21, and Nipponbare, was 78.3%, 77.5%, 74.2%, 75.3%, 70.9%, and 72.1%, respectively.