ALTERNATIVE RNA SPLICING GENERATES TRANSCRIPTS ENCODING A THORAX-SPECIFIC ISOFORM OF DROSOPHILA-MELANOGASTER MYOSIN HEAVY-CHAIN
ALTERNATIVE RNA SPLICING GENERATES TRANSCRIPTS ENCODING A THORAX-SPECIFIC ISOFORM OF DROSOPHILA-MELANOGASTER MYOSIN HEAVY-CHAIN
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DOI：
10.1128/mcb.6.7.2511
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发表时间：
1986-07-01
影响因子：
5.3
通讯作者：
EMERSON, CP
中科院分区：
文献类型：
--
作者：
BERNSTEIN, SI;HANSEN, CJ;EMERSON, CP
基因组学和cDNA测序研究表明，果蝇肌肉肌球蛋白重链（MHC）基因的转录本是选择性剪接的，产生的RNA编码至少两种具有不同C末端的MHC亚型。编码具有27个独特C末端氨基酸的MHC亚型的转录本在幼虫和成虫肌肉分化期间积累。第二种亚型的转录本编码一个独特的C-末端氨基酸，并且几乎完全在蛹和成虫的胸段（间接飞行肌肉的位置）中编码。胸特异性外显子前的3“剪接受体位点异常富含嘌呤，因此可能作为胸特异性剪接信号。我们认为，这两种MHC亚型的C末端控制肌丝组装，并可能在产生飞行肌和其他肌肉类型的独特肌丝组织中发挥作用。
Genomic and cDNA sequencing studies show that transcripts from the muscle myosin heavy-chain (MHC) gene of Drosophila melanogaster are alternatively spliced, produced RNAs that encode at least two MHC isoforms with different C termini. Transcripts encoding an MHC isoform with 27 unique C-terminal amino acids accumulate during both larval and adult muscle differentiation. Transcripts for the second isoform encode one unique C-terminal amino acid and accumualte almost exclusively in pupal and adult thoracic segments, the location of the indirect flight muscles. The 3'' splice acceptor site preceding the thorax-specific exon is unusually purine rich and thus may serve as a thorax-specific splicing signal. We suggest that the alternative C termini of these two MHC isoforms control myofilament assembly and may play a role in generating the distinctive myofilament orgnaiations of flight muscle and other muscle types.