Functional genomic analysis of cell division in C-elegans using RNAi of genes on chromosome III
Functional genomic analysis of cell division in C-elegans using RNAi of genes on chromosome III
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DOI：
10.1038/35042526
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发表时间：
2000-11-16
期刊：
影响因子：
64.8
通讯作者：
Hyman, AA
中科院分区：
文献类型：
--
作者：
Gönczy, P;Echeverri, C;Hyman, AA
基因组测序项目产生了丰富的信息；然而，这些项目的最终目标是加速识别基因的生物学功能。这就需要全面的研究来填补序列和功能之间的空白。在这里，我们报告了功能基因组筛选的结果，以鉴定秀丽隐杆线虫细胞分裂所需的基因。我们使用rna介导的干扰（RNAi）抑制了III号染色体上类似的2,300个预测开放阅读框中的96%的表达。通过使用体内延时差干涉对比显微镜分析，我们确定了早期胚胎中不同细胞过程所必需的133个基因（接近6%）。我们的结果表明，这些基因代表了秀丽隐杆线虫胚胎中正常细胞分裂所需的III号染色体上的大部分基因。完整的数据集，包括延时录音样本，已存放在一个开放获取的数据库中。我们发现，与差异干扰对比表型相关的47%的基因在其他真核生物中有明确的同源物，这表明该筛选也为其他物种提供了假定的基因功能。
Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of similar to 96% of the similar to2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (similar to6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that similar to 47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.