Contribution of Salmonella typhimurium type III secretion components to needle complex formation
Contribution of Salmonella typhimurium type III secretion components to needle complex formation
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DOI：
10.1073/pnas.200209497
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发表时间：
2000-09-26
影响因子：
11.1
通讯作者：
Miller, SI
中科院分区：
文献类型：
--
作者：
Kimbrough, TG;Miller, SI
prgHIJK操纵子编码鼠伤寒沙门氏菌致病岛1 III型分泌系统（TTSS）的组分。以前，prgH和prgK被证明是形成超分子III型分泌针复合物（NC）所需的[Kubori. T.，等人（1998）Science 280. 602-605]。这项工作表明，所有的prg操纵子基因所需的NC形成。PrgH多聚成一个独特的四聚体形状的结构，这可能是一个早期的中间NC组装，并可能提供所需的PrgK低聚化的结构基础。PrgH和PrgK在不存在其他TTSS组分的情况下，寡聚成在外观和大小上与NC的基部相同的环状结构。表明它们可能是分泌所需的主要内膜结构组分。PrgI和PrgJ与NC共片段化并分泌到培养上清液NC中，来自prgI和prgJ突变体的PrgI和PrgJ具有与跨链（基体）NC组分相同的形态学，但缺少外针，表明PrgI和PrgJ是完整NC组装所需的，并且可能是外针的组分。因此。PrgI和PrgJ通过NC基体分泌。由PrgH/K和InvG/H环组成，参与NC更远端组件的组装。
The prgHIJK operon encodes components of the Salmonella typhimurium pathogenicity island 1 type III secretion system (TTSS). Previously, prgH and prgK were shown to be required for formation of the supramolecular type III secretion needle complex (NC) [Kubori. T., et al. (1998) Science 280. 602-605]. This work indicates that all prg operon genes are required for NC formation. PrgH multimerizes into a distinct tetrameric-shaped structure that may be an early intermediate of NC assembly and may provide the structural foundation required for PrgK oligomerization. PrgH and PrgK, in the absence of other TTSS components, oligomerize into ring-shaped structures identical in appearance and size to the base of the NC. indicating that they are likely the major inner membrane structural components required for secretion. PrgI and PrgJ cofractionate with the NC and are secreted into the culture supernatant NC from prgI and prgJ mutants have an identical morphology to the envelope-spanning (basal body) NC components, but are missing the external needle, indicating that PrgI and PrgJ are required for full NC assembly and are likely components of the external needle. Therefore. PrgI and PrgJ are secreted through the NC basal body. composed in part of PrgH/K and InvG/H rings, to participate in assembly of the more distal components of the NC.