Identification of the candidate genes regulated by RNA-directed DNA methylation in Arabidopsis
Identification of the candidate genes regulated by RNA-directed DNA methylation in Arabidopsis
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DOI：
10.1016/j.bbrc.2008.09.046
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发表时间：
2008-11-21
影响因子：
3.1
通讯作者：
Seki, Motoaki
中科院分区：
文献类型：
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作者：
Kurihara, Yukio;Matsui, Akihifo;Seki, Motoaki
RNA指导的DNA甲基化（RdDM）是其中24个核苷酸（nt）的小干扰RNA（siRNA）指导同源基因组DNA区域中的从头胞嘧啶甲基化的过程。在涉及24 nt siRNA积累的几个因素中，RNA依赖性RNA聚合酶2（RDR 2）是关键组分。因为在拟南芥rdr 2突变体中24 nt siRNA的积累消失。在这里，我们比较了野生型，rdr 2 -1和ddc（drm 1drm 2cmt 3），DNA甲基转移酶三重突变体的表达谱，使用全基因组拼接阵列，以确定候选基因直接下调RdDM相关的24 nt siRNA。在突变体中上调的转录物中，我们寻找其编码区或侧翼区具有siRNA产生基因座的转录物。我们发现上调表达的18个转录AGI代码和19个预测的转录单位（TU）与siRNA位点在两个RDR 2 -1和DDC。我们的研究为进一步了解RdDM与候选基因的关系提供了重要信息。(c)2008年爱思唯尔公司All rights reserved.
RNA-directed DNA methylation (RdDM) is a process in which 24 nucleotide (nt) small interfering RNAs (siRNAs) guide de novo cytosine methylation in the homologous genomic DNA region. Of several factors involving 24 nt siRNA accumulation, RNA-dependent RNA polymerase 2 (RDR2) is a key component. because accumulation of 24 nt siRNA disappears in the Arabidopsis rdr2 mutant. Here, we compared expression profiles among wild-type, rdr2-1 and ddc (drm1drm2cmt3), DNA methyltransferase triple mutant, using a whole genome tiling array to identify the candidate genes directly downregulated by RdDM-related 24 nt siRNAs. Of the transcripts upregulated in the Mutants, we searched for those whose coding regions or flanking regions have siRNA-generating loci. We found upregulated expression of 18 transcripts with AGI codes and 19 predicted transcriptional units (TUs) with siRNA loci in both rdr2-1 and ddc. Our study provided important information for understanding the relationship between RdDM and the identified candidate genes. (c) 2008 Elsevier Inc. All rights reserved.