Isolation and physiological characterization of Syntrophus buswellii strain GA from a syntrophic benzoate degrading strictly anaerobic coculture
Isolation and physiological characterization of Syntrophus buswellii strain GA from a syntrophic benzoate degrading strictly anaerobic coculture
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DOI：
10.1007/bf00164509
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发表时间：
1995-12-01
影响因子：
5
通讯作者：
Winter, J
中科院分区：
文献类型：
--
作者：
Auburger, G;Winter, J
一个稳定的，互养苯甲酸降解菌群从污水污泥中富集。它将苯甲酸盐或3-苯基丙酸盐氧化为乙酸盐、H-2和CO2。作为氢清除剂，存在亨氏甲烷菌和脱硫弧菌。这种互养培养物和巴氏互养菌的苯甲酸盐降解细菌能够在没有氢利用伴侣生物的情况下与苯甲酸盐/巴豆酸盐或单独的巴豆酸盐一起生长。如果巴豆酸盐是唯一的底物，则产生乙酸盐和丁酸盐，而在苯甲酸盐或3-苯基丙酸盐上生长期间，巴豆酸盐充当可还原的共底物，并且专门转化为丁酸盐。在巴豆酸的存在下，种间氢转移是没有必要的氢汇。苯甲酸降解菌分离为纯培养物，巴豆酸作为唯一的碳源。纯培养物也可以与苯甲酸/巴豆酸或3-苯基丙酸/巴豆酸一起生长。研究了高浓度巴豆酸盐和乙酸盐或丁酸盐对苯甲酸盐降解菌生长的影响。并与S. buswellii的形态、生理和DNA碱基组成。除了S。Buswellii也能够在肉桂酸盐上生长，在两种生物体之间没有检测到差异。该分离株命名为S. buswellii、GA.
A stable, syntrophic benzoate-degrading bacterial consortium was enriched from sewage sludge. It oxidized benzoate or 3-phenylpropionate to acetate, H-2 and CO2. As hydrogen scavengers Methanospirillum hungatei and Desulfovibrio sp. were present. The benzoate-degrading bacteria of this syntrophic culture and of Syntrophus buswellii were able to grow with benzoate/crotonate or crotonate alone in the absence of a hydrogen-utilizing partner organism. If crotonate was the only substrate, acetate and butyrate were produced, while during growth on benzoate or 3-phenylpropionate crotonate served as a reducible co-substrate and was exclusively converted to butyrate. In the presence of crotonate interspecies hydrogen transfer was not necessary as a hydrogen sink. The benzoate degrader was isolated as a pure culture with crotonate as the only carbon source. The pure culture could also grow with benzoate/crotonate or 3-phenylpropionate/crotonate. The effect of high concentrations of crotonate and of acetate or butyrate on growth of the benzoate degrader was investigated. The benzoate degrader was compared with S. buswellii for its morphology, physiology and DNA base composition. Except for the fact that S. buswellii was also able to grow on cinnamate, no differences between the two organisms were detected. The isolate is named S. buswellii, strain GA.