Three-Dimensional Engineered Bone-Ligament-Bone Constructs for Anterior Cruciate Ligament Replacement
Three-Dimensional Engineered Bone-Ligament-Bone Constructs for Anterior Cruciate Ligament Replacement
复制标题
DOI：
10.1089/ten.tea.2011.0231
复制
发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
Arruda, Ellen M.
中科院分区：
文献类型：
--
作者：
Ma, Jinjin;Smietana, Michael J.;Arruda, Ellen M.
前交叉韧带（ACL）是膝关节的主要稳定器，通常会受伤。由于其固有的愈合能力差，撕裂的ACL通常通过移植物重建。我们使用骨髓基质细胞和绵羊作为模型系统，开发了一种用于ACL移植物的多相或骨-韧带-骨组织工程结构。在体内6个月后，结构的横截面增加，并表现出组织良好的微观结构，天然骨整合，功能性附着点，血管化，神经支配，增加胶原蛋白含量和结构对齐。结构的刚度增加到52%的切线模量和95%的原生ACL的几何刚度。外植体的粘弹性反应与成人ACL几乎没有区别。这些结果表明，我们的结构植入后，可以获得生理相关的结构和功能特性的成人ACL。它们为ACL置换提供了一种可行的选择。
The anterior cruciate ligament (ACL), a major stabilizer of the knee, is commonly injured. Because of its intrinsic poor healing ability, a torn ACL is usually reconstructed by a graft. We developed a multi-phasic, or bone-ligament-bone, tissue-engineered construct for ACL grafts using bone marrow stromal cells and sheep as a model system. After 6 months in vivo, the constructs increased in cross section and exhibited a well-organized microstructure, native bone integration, a functional enthesis, vascularization, innervation, increased collagen content, and structural alignment. The constructs increased in stiffness to 52% of the tangent modulus and 95% of the geometric stiffness of native ACL. The viscoelastic response of the explants was virtually indistinguishable from that of adult ACL. These results suggest that our constructs after implantation can obtain physiologically relevant structural and functional characteristics comparable to those of adult ACL. They present a viable option for ACL replacement.