TRANSCRIPTION, ORGANIZATION, AND SEQUENCE OF AN AUXIN-REGULATED GENE-CLUSTER IN SOYBEAN
TRANSCRIPTION, ORGANIZATION, AND SEQUENCE OF AN AUXIN-REGULATED GENE-CLUSTER IN SOYBEAN
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DOI：
10.1105/tpc.1.2.229
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发表时间：
1989-02-01
期刊：
影响因子：
11.6
通讯作者：
GUILFOYLE, TJ
中科院分区：
文献类型：
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作者：
MCCLURE, BA;HAGEN, G;GUILFOYLE, TJ
我们已经确定了一个大豆基因簇，它编码一组生长素调控的RNA（小生长素上调RNA）。基因座的一部分的DNA测序揭示了五个同源基因，间隔约1.25个碱基，并在交替方向转录。这些基因中至少有三个受生长素的转录调控。在将生长素应用于大豆伸长下胚轴切片后10分钟检测到转录速率的增加。每个基因都包含一个开放的阅读框架，可以编码9千道尔顿至10.5千道尔顿的蛋白质。五个基因之间的序列比较揭示了几个高度同源性的领域。两个高同源性区域开始于开放阅读框上游约250个碱基对，并且在开放阅读框下游的序列中鉴定了两个同源性区域。后者序列之一出现在RNA的3“-非翻译区。另一个发生在远下游，618碱基对至741碱基对形成终止密码子。这些序列在五个不同基因中的保守性表明它们可能对基因表达的调节很重要。
We have characterized a soybean gene cluster that encodes a group of auxin-regulated RNAs (small auxin up RNAs). DNA sequencing of a portion of the locus reveals five homologous genes, spaced at intervals of about 1.25 kilobases and transcribed in alternate directions. At least three of the genes are transcriptionally regulated by auxin. An increase in the rate of transcription is detected 10 min after application of auxin to soybean elongating hypocotyl sections. Each of the genes contains an open reading frame that could encode a protein of 9 kilodaltons to 10.5 kiolodaltons. Sequence comparisons among the five genes reveal several areas of high homology. Two regions of high homology begin about 250 base pairs upstream of the open reading frames and two regions of homology have been identified in sequences downstream of the open reading frames. One of the latter sequences occurs in the 3''-untranslated region of the RNAs. The other occurs far downstream, 618 base pairs to 741 base pairs form the stop codon. Conservation of these squences among the five different genes suggests that they may be important for the regulation of expression of the genes.