Differential expression of microRNA species in organs of hibernating ground squirrels: A role in translational suppression during torpor
Differential expression of microRNA species in organs of hibernating ground squirrels: A role in translational suppression during torpor
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DOI：
10.1016/j.bbagrm.2008.07.011
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发表时间：
2008-10-01
影响因子：
4.7
通讯作者：
Storey, Kenneth B.
中科院分区：
文献类型：
--
作者：
Morin, Pier Jr.;Dubuc, Adrian;Storey, Kenneth B.
哺乳动物的冬眠包括长时间的深度麻木，其中所有代谢过程的速率都以可逆的方式受到强烈抑制。我们假设 microRNA（miRNA），即与 mRNA 结合的小型非编码转录物，在动物进入冬眠状态时，可能在 mRNA 翻译的整体抑制中发挥作用。使用 RT-PCR 在常温与冬眠地松鼠（Spermophilus tridecemlineatus）的四个器官中评估选定的 miRNA 种类（以下 4-9 个：mir-1、mir-24、mir-15a、mir-16、mir-21、mir-122a、mir-143、mir-146 和 mir-206）。在迟钝动物的心脏和骨骼肌中，mir-24 转录物的水平显着降低，肌肉中的 mir-122a 水平也显着降低。休眠期间，肾脏中的 Mir-1 和 mir-21 均显着增加，分别增加了 2.0 倍和 1.3 倍。在肝脏中分析的四种 miRNA 没有发现变化。 Dicer（一种参与 miRNA 加工的酶）的蛋白质水平也在心脏、肾脏和肝脏中进行了定量。冬眠期间心脏中的 Dicer 蛋白水平增加了 2.7 倍，但肾脏中的 Dicer 蛋白水平下降了 60%。这些数据是第一份关于哺乳动物冬眠期间 miRNA 水平发生差异调节的报告，它们提供了一种在冬眠期间可逆基因沉默的机制，当动物醒来回到正常体温时，这种沉默可以迅速逆转，从而允许 mRNA 的重新翻译。 (C) 2008 Elsevier B.V. 保留所有权利。
Mammalian hibernation includes long periods of profound torpor where the rates of all metabolic processes are strongly suppressed in a reversible manner. We hypothesized that microRNAs (miRNAs), small noncoding transcripts that bind to mRNA, could play a role in the global suppression of mRNA translation when animals enter torpor. Selected miRNA species (4-9 of the following: mir-1, mir-24, mir-15a. mir-16, mir-21, mir-122a, mir-143, mir-146 and mir-206) were evaluated in four organs of euthermic versus hibernating ground squirrels, Spermophilus tridecemlineatus using RT-PCR. Levels of mir-24 transcripts were significantly reduced in heart and skeletal muscle of torpid animals as were mir-122a levels in the muscle. Mir-1 and mir-21 both increased significantly in kidney during torpor by 2.0- and 1.3-fold, respectively. No changes were found for the four miRNA species analyzed in liver. Protein levels of Dicer, an enzyme involved in miRNA processing were also quantified in heart, kidney and liver. Dicer protein levels increased by 2.7-fold in heart during hibernation but decreased by 60% in kidney. These data are the first report that differential regulation of miRNA levels Occurs during mammalian hibernation and they provide a mechanism for reversible gene silencing during torpor that can be rapidly reversed to allow renewed translation of mRNA when animals arouse back to euthermia. (C) 2008 Elsevier B.V. All rights reserved.