SRPX2 is overexpressed in gastric cancer and promotes cellular migration and adhesion
SRPX2 is overexpressed in gastric cancer and promotes cellular migration and adhesion
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DOI：
10.1002/ijc.24065
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发表时间：
2009-03-01
影响因子：
6.4
通讯作者：
Nishio, Kazuto
中科院分区：
文献类型：
--
作者：
Tanaka, Kaoru;Arao, Tokuzo;Nishio, Kazuto
SRPX 2（Sushi repeat containing protein，X-linked 2）首先在t（17;19）阳性白血病细胞中被鉴定为E2 A-HLF融合基因的下游分子，该基因的生物学功能尚不清楚。我们发现SRPX 2在胃癌中过表达，并且表达和临床特征表明，使用实时RT-PCR在预后不良的患者中观察到高mRNA表达水平。SRPX 2蛋白的细胞分布显示SRPX 2分泌到细胞外区域并定位于细胞质中。SRPX 2基因导入HEK 293细胞中不调节细胞增殖活性，但确实增强细胞迁移活性，如使用迁移和划痕测定所示。从SRPX 2过表达细胞获得的条件培养基增加了胃癌细胞系SNU-16的细胞迁移活性。此外，SRPX 2蛋白显著增强SNU-16和HSC-39的细胞粘附，并增加粘着斑激酶（FAK）的磷酸化水平，如使用蛋白质印迹所示，表明SRPX 2通过FAK信号传导增强细胞迁移和粘附。总之，SRPX 2的过表达增强了胃癌细胞的细胞迁移和粘附。在这里，我们报告SRPX 2的生物学功能包括细胞迁移和粘附癌细胞。(c)2008 Wiley-Liss，Inc.
SRPX2 (Sushi repeat containing protein, X-linked 2) was first identified as a downstream molecule of the E2A-HLF fusion gene in t(17;19)-positive leukemia cells and the biological function of this gene remains unknown. We found that SRPX2 is overexpressed in gastric cancer and the expression and clinical features showed that high mRNA expression levels were observed in patients with unfavorable outcomes using real-time RT-PCR. The cellular distribution of SRPX2 protein showed the secretion of SRPX2 into extracellular regions and its localization in the cytoplasm. The introduction of the SRPX2 gene into HEK293 cells did not modulate the cellular proliferative activity but did enhance the cellular migration activity, as shown using migration and scratch assays. The conditioned-medium obtained from SRPX2-overexpressing cells increased the cellular migration activity of a gastric cancer cell line, SNU-16. In addition, SRPX2 protein remarkably enhanced the cellular adhesion of SNU-16 and HSC-39 and increased the phosphorylation levels of focal adhesion kinase (FAK), as shown using western blotting, suggesting that SRPX2 enhances cellular migration and adhesion through FAK signaling. In conclusion, the overexpression of SRPX2 enhances cellular migration and adhesion in gastric cancer cells. Here, we report that the biological functions of SRPX2 include cellular migration and adhesion to cancer cells. (c) 2008 Wiley-Liss, Inc.