Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth
Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth
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拟南芥叶齿生长素动态与水囊发育的时空关系
DOI：
10.1080/15592324.2021.1989216
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发表时间：
2021
影响因子：
2.9
通讯作者：
Shimada Tomoo
中科院分区：
文献类型：
--
作者：
Yagi Hiroki;Tamura Kentaro;Matsushita Tomonao;Shimada Tomoo
水柱是维管植物的一种组织，参与水的释放，称为管状管。拟南芥位于叶齿的尖端，包含三个主要组成部分：水孔、木质部末端和小细胞。叶齿是植物生长发育过程中生长素合成和积累的主要部位。然而，生长素动态与水螅体发育之间的详细时空关系尚不清楚。在这项研究中，我们发现生长素的生物合成和积累先于水螅体的发育。构建了包含三个叶片标记的三重标记系（YDE系）：YUC4:nls-3xGFP（生长素生物合成）、DR5rev:erRFP（生长素积累或最大值）和E325-GFP（水根发育），并进行了时空共聚焦显微镜分析。在叶片发育过程中，这些标记的表达面积逐渐增大，表明叶柄大小随着叶片的生长而增大。详细观察发现，生长素相关标记YUC4:nls-GFP和DR5rev:erRFP在叶片生长早期首次表达。E325-GFP在后期与生长素标记物部分重叠表达。这些发现为研究拟南芥植物生长素动态与水螅体发育的时空关系提供了新的思路。
Hydathode is a plant tissue of vascular plants involved in water release called guttation.Arabidopsishydathodes are found at the tips of leaf teeth and contain three major components: water pores, xylem ends, and small cells. Leaf teeth are known as the main parts for auxin biosynthesis and accumulation during leaf development. However, the detailed spatiotemporal relationship between auxin dynamics and hydathode development is unknown. In this study, we show that auxin biosynthesis and accumulation precede hydathode development. A triple marker line (called YDE line) containing three leaf tooth markers: YUC4:nls-3xGFP (auxin biosynthesis), DR5rev:erRFP (auxin accumulation or maxima), and E325-GFP (hydathode development), was generated, and spatiotemporal confocal microscopic analysis was carried out. The expression area of these markers became larger during leaf development, implying that the hydathode size enlarges as the leaf tooth grows. Detailed observation revealed that the auxin-related markers YUC4:nls-GFP and DR5rev:erRFP were first expressed in the early stage of leaf tooth growth. Then, E325-GFP was expressed partly overlapping with the auxin markers at a later stage. These findings provide new insights into the spatiotemporal relationship between auxin dynamics and hydathode development inArabidopsis.