INDUCTION AND ACCUMULATION OF MAJOR TUBER PROTEINS OF POTATO IN STEMS AND PETIOLES
INDUCTION AND ACCUMULATION OF MAJOR TUBER PROTEINS OF POTATO IN STEMS AND PETIOLES
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DOI：
10.1104/pp.71.1.161
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发表时间：
1983-01-01
期刊：
影响因子：
7.4
通讯作者：
PARK, WD
中科院分区：
文献类型：
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作者：
PAIVA, E;LISTER, RM;PARK, WD
免疫上相同的糖蛋白家族，表观分子量为。apprx。其中4万个是马铃薯（S. tuberosum L.）的主要块茎蛋白。这些通过离子交换和亲和层析纯化的蛋白质被命名为patatin。为了确定patatin是否可以作为研究结核过程的生化标志物，采用火箭免疫电泳和酶联免疫吸附试验（ELISA）测定了patatin在多种组织中的含量。无论块茎是在地下匍匐茎上形成的，还是在茎插枝的腋芽上形成的，块茎中都含有40-45%的可溶性蛋白。在正常情况下，植物的叶子、茎或根中只有微量的patatin存在，这些植物要么正在积极形成块茎，要么已经长时间生长以防止块茎形成。如果块茎和腋芽被切除，花青素可以在茎和叶柄中积累。这种积累没有任何明显的结节样肿胀，即使长时间也会发生。在所有含有大量patatin的组织中，其他块茎蛋白以及大量淀粉也存在。
A family of immunologically identical glycoproteins with apparent MW of .apprx. 40,000 are among the major tuber proteins of potato (S. tuberosum L.). These proteins, as purified by ion-exchange and affinity chromatography, were given the trivial name patatin. To determine if patatin can be used as a biochemical marker to study the process of tuberization, its amount was measured in a variety of tissues by rocket immunoelectrophoresis and by enzyme-linked immunosorbent assay (ELISA). Patatin comprises 40-45% of the soluble protein in tubers regardless of whether they are formed on underground stolons or from axillary buds of stem cuttings. Under normal conditions, patatin is present in only trace amounts, if at all, in leaves, stems or roots of plants which are either actively forming tubers or which have been grown under long days to prevent tuberization. If tubers and axillary buds are removed, patatin can accumulate in stems and petioles. This accumulation occurred without any obvious tuber-like swelling and would occur even under long days. In all tissues containing large amounts of patatin, the other tuber proteins were also present as well as large amounts of starch.