In vitro growth of pure cultures of retinal pigment epithelium.
In vitro growth of pure cultures of retinal pigment epithelium.
复制标题
视网膜色素上皮纯培养物的体外生长。
DOI：
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发表时间：
1972
期刊：
影响因子：
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通讯作者：
A. Rabson
中科院分区：
文献类型：
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作者：
D. Albert;M. Tso;A. Rabson
视网膜色素上皮细胞（RPE）分离自五个人的眼睛，并在体外生长作为外植体培养。同样建立脉络膜和RPE与脉络膜的培养物用于比较。将组织保持在培养物中长达六个月，并研究和记录细胞的形态特征。镶嵌，管状，纺锤形生长模式被观察到从RPE演变。某些细胞学特征提示去分化和瘤形成。随着组织培养的进行，细胞中的色素量普遍减少。将在组织培养中生长的细胞的外观与在正常和病理情况下观察到的RPE的外观进行比较。
Retinal pigment epithelium (RPE) was isolated from five human eyes and grown in vitro as an explant culture. Cultures of choroid and RPE with choroid were similarly established for comparison. The tissues were maintained in culture for periods up to six months, and the morphologic characteristics of the cells were studied and documented. Mosaic, tubular, and spindle growth patterns were observed to evolve from the RPE. Certain cytologic features suggestive of dedifferentiation and neoplasia were noted. There was a general decrease in the amount of pigment in the cells as tissue culture progressed. The appearance of the cells grown in tissue culture is compared with that of RPE seen in normal and pathologic situations.