Oral Microbiota Perturbations Are Linked to High Risk for Rheumatoid Arthritis
Oral Microbiota Perturbations Are Linked to High Risk for Rheumatoid Arthritis
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口腔微生物群扰动与类风湿性关节炎的高风险有关
DOI：
10.3389/fcimb.2019.00475
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发表时间：
2020-01-22
影响因子：
5.7
通讯作者：
Liu, Yi
中科院分区：
文献类型：
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作者：
Tong, Yanli;Zheng, Linlin;Liu, Yi
已知口腔微生物生态失调会增加个体发展为类风湿性关节炎（RA）的易感性。有RA风险的个体可能经历疾病进展的不同阶段。在这项研究中，我们的目的是调查口腔微生物群落是否以及如何在RA症状出现之前发生变化。收集了29名高风险个体的79份唾液样本，这些个体抗瓜氨酸蛋白抗体（ACPA）呈阳性，并且没有临床关节炎，27名RA患者和23名健康对照（HC）。使用16 S核糖体RNA基因测序检查唾液微生物组。分别采用α和β多样性分析和线性判别分析对3个类群的细菌多样性、群落结构和判别类群进行了分析。分析唾液细菌与自身抗体的相关性。在“临床前”阶段，唾液微生物多样性显着减少相比，类风湿关节炎患者和HC。与HC相反，与RA患者一样，RA高危个体显示出Defluviitaleaceae_UCG-011属和口腔奈瑟氏菌物种丰度的减少，但Prevotella_6的扩增。出乎意料的是，牙龈卟啉单胞菌的相对丰度，报告为RA发展的机会致病菌，在高危人群中显着下降。此外，我们确定了四个属的唾液中的高危人群与血清ACPA滴度呈正相关，和其他两个属呈负相关。总之，我们观察到RA高危个体唾液微生物的特征性组成变化，表明口腔微生物群失调发生在RA的“临床前”阶段，并与全身性自身免疫特征相关。
Oral microbial dysbiosis is known to increase susceptibility of an individual to develop rheumatoid arthritis (RA). Individuals at-risk of RA may undergo different phases of disease progression. In this study, we aim to investigate whether and whereby the oral microbiome communities alter prior to symptoms of RA. Seventy-nine saliva samples were collected from 29 high-risk individuals, who were positive for anti-citrullinated protein antibodies (ACPA) and have no clinical arthritis, 27 RA patients and 23 healthy controls (HCs). The salivary microbiome was examined using 16S ribosomal RNA gene sequencing. Alpha and beta diversity analysis and the linear discriminant analysis were applied to examine the bacterial diversity, community structure and discriminatory taxa between three groups, respectively. The correlation between salivary bacteria and autoantibodies were analyzed. In the "pre-clinical" stages, salivary microbial diversity was significantly reduced comparing to RA patients and HCs. In contrast to HCs, like RA patients, individuals at high-risk for RA showed a reduction in the abundance of genus Defluviitaleaceae_UCG-011 and the species Neisseria oralis, but an expansion of Prevotella_6. Unexpectedly, the relative abundance of Porphyromonas gingivalis, reported as opportunistic pathogens for RA development, was significantly decreased in high-risk individuals. Additionally, we identified four genera in the saliva from high-risk individuals positively correlated with serum ACPA titers, and the other two genera inversely displayed. In summary, we observed a characteristic compositional change of salivary microbes in individuals at high-risk for RA, suggesting that oral microbiota dysbiosis occurs in the "pre-clinical" stage of RA and are correlated with systemic autoimmune features.