Surgical microscope with integrated fluorescence lifetime imaging for 5-aminolevulinic acid fluorescence-guided neurosurgery
Surgical microscope with integrated fluorescence lifetime imaging for 5-aminolevulinic acid fluorescence-guided neurosurgery
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DOI：
10.1117/1.jbo.25.7.071202
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发表时间：
2020-07-01
影响因子：
3.5
通讯作者：
Andreana, Marco
中科院分区：
文献类型：
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作者：
Erkkila, Mikael T.;Reichert, David;Andreana, Marco
重要性：传统神经外科显微镜中基于5-氨基乙酰丙酸（5-ALA）的荧光引导仅限于强荧光肿瘤组织。因此，更灵敏的，intrasurgical 5-ALA荧光visualizationisneed.Aim：宏观荧光寿命成像（FLIM）进行离体5-ALA标记的人脑胶质瘤组织通过手术显微镜，以评估其可行性，并将其与荧光强度imaging. Approach：频域FLIM集成到一个手术显微镜，这使得并行宽视场白光和荧光成像。我们首先描述了我们的系统，并对两个疑似低级别胶质瘤的样本进行了成像，并与组织病理学进行了比较。结果：我们的成像系统能够以空间分辨率实现6.5 x 6.5 mm（2）视野的宏观FLIM
Significance: 5-Aminolevulinic acid (5-ALA)-based fluorescence guidance in conventional neurosurgical microscopes is limited to strongly fluorescent tumor tissue. Therefore, more sensitive, intrasurgical 5-ALA fluorescence visualization is needed.Aim: Macroscopic fluorescence lifetime imaging (FLIM) was performed ex vivo on 5-ALA-labeled human glioma tissue through a surgical microscope to evaluate its feasibility and to compare it to fluorescence intensity imaging.Approach: Frequency-domain FLIM was integrated into a surgical microscope, which enabled parallel wide-field white-light and fluorescence imaging. We first characterized our system and performed imaging of two samples of suspected low-grade glioma, which were compared to histopathology.Results: Our imaging system enabled macroscopic FLIM of a 6.5 x 6.5 mm(2) field of view at spatial resolutions