Biofilm formation on rat skin wounds by Pseudomonas aeruginosa carrying the green fluorescent protein gene
Biofilm formation on rat skin wounds by Pseudomonas aeruginosa carrying the green fluorescent protein gene
复制标题
携带绿色荧光蛋白基因的铜绿假单胞菌在大鼠皮肤伤口上形成生物膜
DOI：
10.1111/j.1600-0625.2009.00931.x
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发表时间：
2010-02-01
影响因子：
3.6
通讯作者：
Tachi, Masahiro
中科院分区：
文献类型：
--
作者：
Kanno, Emi;Toriyabe, Souhachi;Tachi, Masahiro
大多数慢性伤口被生物膜覆盖。然而，关于生物膜是否是延迟伤口愈合的致病因素以及生物膜状态是否有助于这种病理学的问题仍然存在。本研究的目的是建立一种便于观察皮肤创面生物膜形成的实验模型。在SD大鼠背部造成全层创伤。然后将携带编码绿色荧光蛋白基因的铜绿假单胞菌悬浮液应用于伤口。分别于伤后8 h、1、3、7 d取材，行组织学和免疫组化检查。荧光显微镜检查证实早在8小时就存在生物膜。与非感染伤口相比，上皮形成没有延迟。总之，大鼠急性伤口的伤口愈合不受生物膜形成的影响。
Most chronic wounds are covered by biofilms. However, questions remain about whether biofilms are a causative factor in delayed wound healing and whether the biofilm state contributes to this pathology. The purpose of this study was to develop an experimental model for convenient observation of biofilm formation on skin wounds. Full-thickness wounds were created on the backs of SD rats. Suspensions of Pseudomonas aeruginosa carrying the gene encoding green fluorescent protein were then applied to the wounds. The wounds were harvested at 8 h, and at 1, 3 and 7 days postwounding for histological and immunohistochemical examinations. Fluorescence microscopy confirmed the presence of a biofilm as early as 8 h. Comparing with non-infected wounds, epithelialization was not delayed. In conclusion, wound healing of rat acute wounds was unaffected by biofilm formation.