Enterobacteria Phage SV76 Host Range and Genomic Characterization.
Enterobacteria Phage SV76 Host Range and Genomic Characterization.
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DOI：
10.1089/phage.2022.0005
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Nugen, Sam R.
中科院分区：
文献类型：
--
作者：
Carmody, Caitlin M.;Farquharson, Emma L.;Nugen, Sam R.
增加噬菌体基因组数据的数量和细节对于扩大我们对噬菌体如何运作的理解至关重要，从而使我们能够利用其独特的特性促进生物技术的进步。在这里，我们提出了完整的序列噬菌体SV 76的组装和注释的基因组（登录号OM 339528）。SV 76先前已被分类为T4样噬菌体，属于收缩尾噬菌体的肌尾病毒科（Myoviridae）中的Tequatrovirus属。对SV76进行全基因组测序、组装和注释。利用双琼脂斑点试验确定SV76对一组72株大肠杆菌分离株的宿主范围，以代表大肠杆菌的多样性。大肠杆菌，以及一系列的敲除设计，以确定所需的受体结合蛋白。将基因组和宿主范围与密切相关的噬菌体T2进行比较。斑点试验表明，SV76能在72株大肠杆菌中的10株（13.9%）和9株大肠杆菌中形成空斑。coli K12单基因敲除已知噬菌体受体（100%）。SV76不能在ΔfadL E上铺板。表明需要作为受体结合蛋白。SV76与T2密切相关，在ECOR内具有相似的宿主范围。这项研究提供了SV76噬菌体的新宿主范围和基因组数据，为未来进一步表征SV76的研究提供了基础，以了解更多关于SV76和其他T4样噬菌体的信息，这些噬菌体可用于创造新的生物技术。
Increasing the quantity and detail of bacteriophage genomic data is critical to broadening our understanding of how bacteriophages operate to allow us to harness their unique properties for biotechnology advancements. Here we present the complete sequence of phage SV76’s assembled and annotated genome (Accession OM339528). SV76 has previously been classified as a T4-like bacteriophage belonging to the Tequatrovirus genus within the Myoviridae family of contractile tailed bacteriophages. Whole genome sequencing, assembly, and annotation was performed on SV76. Double-agar spot assays were utilized to determine SV76’s host range against a panel of 72 Escherichia coli isolates meant to represent the diversity of E. coli, as well as a series of knockouts designed to identify required receptor binding proteins. The genome and host range were compared to the closely related phage, T2. Spot assays revealed that SV76 could plaque on 10 of the 72 strains (13.9 %) and nine of the nine E. coli K12 single gene knockout of known phage receptors (100%). SV76 did not plate on a ΔfadL E. coli indicating suggesting a requirement as a receptor binding protein. SV76 is closely related to T2 with similar host ranges within ECOR. This study presents novel host range and genomic data on SV76 phage, providing a foundation for future studies to further characterize SV76 to understand more about SV76 and other T4-like phages that can be applied to create novel biotechnologies.
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影响因子：
14.9
作者：
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