Antibacterial activities of β-lactamase inhibitors associated with morphological changes of cell wall in Helicobacter pylori
Antibacterial activities of β-lactamase inhibitors associated with morphological changes of cell wall in Helicobacter pylori
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DOI：
10.1046/j.1523-5378.2002.00054.x
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发表时间：
2002-02-01
期刊：
影响因子：
4.4
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
--
作者：
Horii, T;Mase, K;Kobayashi, M
背景近年来的研究表明，β-内酰胺酶抑制剂包括克拉维辛、舒巴坦和他唑巴坦对幽门螺杆菌具有体外抗菌作用。在这里，我们描述了H。幽门螺杆菌暴露于β-内酰胺酶抑制剂和一些抗生素在短期内的过程。材料和方法。研究了克拉维他、舒巴坦和他唑巴坦3种β-内酰胺酶抑制剂对H. pylori ATCC 43504进行检测。β-内酰胺酶抑制剂如克拉维胺和舒巴坦单独使用可降低H.幽门螺杆菌，取决于抗生素浓度。暴露于这些β-内酰胺酶抑制剂导致细胞形状、细胞壁崩解和细胞溶解的形态学变化。在这些β-内酰胺酶抑制剂中，棒菌素活性最高，可导致活菌计数减少和形态学变化，如短丝状体到球质体形成和溶解。与阿莫西林或克拉维酸单用相比，1 × MIC阿莫西林+1 × MIC克拉维酸可有效降低活菌数，并引起细胞形态和细胞壁的变化。我们的研究结果表明，β-内酰胺酶抑制剂单独对H. pylori感染并影响细胞形态和细胞壁。
Background. Recent study has demonstrated that beta-lactamase inhibitors including clavulanate, sulbactam and tazobactam have an vitro antibacterial effect on Helicobacter pylori. Here we describe the relationship between viability and cell profiles of H. pylori exposed to beta-lactamase inhibitors and some antibiotics in a short-time course.Materials and methods. The antibacterial effects of beta-lactamase inhibitors including clavulanate, sulbactam and tazobactam on the bacterial viability of and morphological changes in H. pylori ATCC43504 were examined.Results. The beta-lactamase inhibitors such as clavulanate and sulbactam alone decreased the viable counts of H. pylori, depending on the antibiotic concentrations. Exposure to these beta-lactamase inhibitors resulted in morphological changes of cell shape, cell-wall disintegration and cell lysis. Among these beta-lactamase inhibitors, clavulanate was the most active, causing a decrease in viable counts and morphological changes such as short filamentous to sphaeroplast formation and lysis. One x minimum inhibitory concentration (MIC) of amoxicillin plus 1 x MIC of clavulanate decreased viable counts effectively compared with 1 x MIC of amoxicillin or 1 x MIC of clavulanate alone, and induced morphological changes of cell shape and cell wall.Conclusion. Our results suggest that the beta-lactamase inhibitors alone have concentration-dependent antibacterial activities against H. pylori and affect the morphology of the cell shape and the cell wall in vitro.