Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia
Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia
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DOI：
10.4012/dmj.28.461
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发表时间：
2009-07-01
影响因子：
2.5
通讯作者：
Bans, Seiji
中科院分区：
文献类型：
--
作者：
Yamashita, Daisuke;Machigashira, Miho;Bans, Seiji
本研究的目的是评价表面粗糙度对小鼠成骨样细胞在CeO_2/Al_2O_3纳米复合材料(NANOZR)和Y_2O_3稳定的氧化锆(3Y-TZP)上初始附着的影响，并与纯钛(Ti)和氧化铝(AO)进行比较。分别用金刚石纸研磨和喷砂制备了光滑表面和粗糙表面的样品。在四种底物中，粗糙表面的附着细胞数显著高于光滑表面的细胞(p<；0.05)。整合素α(5)和β(1)在粗糙表面标本中的表达增加幅度大于光滑表面标本。然而，在光滑和粗糙的表面标本中，肌动蛋白的细胞骨架组织是相似的。NANOZR和3Y-TZP具有良好的细胞贴壁性，与钛和AO相似。结果表明，表面粗糙的NANOZR和3Y-TZP可以提供良好的初始细胞响应，适合于将来的种植体使用。
The aim of this study was to evaluate the effect of surface roughness on the initial attachment of mouse osteoblast-like cells on ceria-stabilized zirconia/alumina nanocomposite (NANOZR) and yttria-stabilized zirconia (3Y-TZP) in comparison to those on pure titanium (Ti) and alumina oxide (AO). Specimens with smooth and rough surfaces were prepared by grinding with diamond paper or by sandblasting, respectively. For four substrates examined, the number of attached cells on the rough surface specimens was significantly higher than that on the smooth surface specimens (p < 0.05). Integrin alpha(5) and beta(1) expression had a greater increase in rough surface specimens than in smooth surface specimens. Actin cytoskeleton organization was, however, similar for both smooth and rough surface specimens. NANOZR and 3Y-TZP produced good cell attachment, similar to Ti and AO. The overall results demonstrated that NANOZR and 3Y-TZP with rough surface could provide good initial cell responses, adequate for future implant usage.