Changes in Bacterial Community Structure of Agricultural Land Due to Long-Term Organic and Chemical Amendments
Changes in Bacterial Community Structure of Agricultural Land Due to Long-Term Organic and Chemical Amendments
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DOI：
10.1007/s00248-012-0025-y
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发表时间：
2012-08-01
影响因子：
3.6
通讯作者：
Nautiyal, Chandra Shekhar
中科院分区：
文献类型：
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作者：
Chaudhry, Vasvi;Rehman, Ateequr;Nautiyal, Chandra Shekhar
采用群落水平的生理分析和基于V1-V2 16 S rRNA基因区的焦磷酸测序分析，对化学耕作地（CCL）、有机耕作地（OCL）和休闲草地（FGL）3种不同土地利用方式下16年的土壤微生物群落结构、多样性和细菌发生进行了表征和比较。整个数据集由16，608个高质量序列组成（CCL，6，379; OCL，4，835; FGL，5，394）;其中12，606个序列可归类于15个已知门。最丰富的门是变形菌门（29.8%），酸菌门（22.6%），放线菌门（11.1%）和拟杆菌门（4.7%），而24.3%的序列来自细菌结构域，但不能进一步分类到任何已知的门。变形菌，拟杆菌，芽单胞菌被发现是显着丰富的OCL土壤。相反，放线菌和酸杆菌在CCL和FGL中分别显着丰富。我们的研究结果支持了这样的观点，即有机堆肥改良剂（OCL）激活不同的微生物组相比，常规使用的合成化学肥料。基于碳源利用模式的功能多样性和均匀度，OCL显著高于CCL和FGL，表明土壤质量有所改善。这种丰富的微生物可能会导致土壤有机碳，土壤有机氮，和微生物生物量的OCL和FGL土壤与CCL整理的水平提高。这项工作增加了我们目前对长期有机和化学改良剂应用对土壤中细菌群落的丰度、多样性和组成的影响的理解，从而为农业产量和土壤数量的前景提供了依据。
Community level physiological profiling and pyrosequencing-based analysis of the V1-V2 16S rRNA gene region were used to characterize and compare microbial community structure, diversity, and bacterial phylogeny from soils of chemically cultivated land (CCL), organically cultivated land (OCL), and fallow grass land (FGL) for 16 years and were under three different land use types. The entire dataset comprised of 16,608 good-quality sequences (CCL, 6,379; OCL, 4,835; FGL, 5,394); among them 12,606 sequences could be classified in 15 known phylum. The most abundant phylum were Proteobacteria (29.8%), Acidobacteria (22.6%), Actinobacteria (11.1%), and Bacteroidetes (4.7%), while 24.3% of the sequences were from bacterial domain but could not be further classified to any known phylum. Proteobacteria, Bacteroidetes, and Gemmatimonadetes were found to be significantly abundant in OCL soil. On the contrary, Actinobacteria and Acidobacteria were significantly abundant in CCL and FGL, respectively. Our findings supported the view that organic compost amendment (OCL) activates diverse group of microorganisms as compared with conventionally used synthetic chemical fertilizers. Functional diversity and evenness based on carbon source utilization pattern was significantly higher in OCL as compared to CCL and FGL, suggesting an improvement in soil quality. This abundance of microbes possibly leads to the enhanced level of soil organic carbon, soil organic nitrogen, and microbial biomass in OCL and FGL soils as collated with CCL. This work increases our current understanding on the effect of long-term organic and chemical amendment applications on abundance, diversity, and composition of bacterial community inhabiting the soil for the prospects of agricultural yield and quantity of soil.