Regulation of motility in sperm of the red-spotted newt.
Regulation of motility in sperm of the red-spotted newt.
复制标题
红斑蝾螈精子活力的调节。
DOI：
10.1002/jez.1402400313
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dent,JN
中科院分区：
文献类型：
--
作者：
Hardy,MP;Dent,JN
在红点蝾螈的输精管、精荚和受精囊中，以及在渗透压、氢离子浓度和特定渗透压物质浓度变化的体外制剂中，对精子的活力进行了体内检查。精子在精荚内是能动的，但在受精囊或输精管中很少或没有看到运动的证据。大约25%的输精管精子在分散在精液血浆（输精管的含精子液体）中时变得活动，表明拥挤可能是一种运动抑制因素。不到50%的运动在几个盐水介质等渗与精子液血浆，而超过90%成为运动在蒸馏水或介质渗透压接近池塘水。等渗溶液中的运动持续超过12小时，但在低渗透压下，运动在6小时后停止。当在较高渗透压下分散时，初始运动性较低，但在12小时时增加至等渗水平。对甘露醇溶液浸泡的反应类似于在等效渗透压的盐溶液中观察到的反应。氢离子浓度在pH 4和9之间的分散并不影响精子的初始活力，但在pH 9下12小时后，pH 4或5的运动受到抑制。在一般情况下，这些数据表明，在存储过程中的强制静止的精子渗透压的主要作用，并表明，低渗透压的池塘水是主要负责激活精子的蝾螈精荚。
The motility of sperm was examined in vivo in the vas deferens, the spermatophore, and the spermatheca of the red‐spotted newt and in in vitro preparations with variations in osmolality, hydrogen ion concentration, and concentrations of specific osmolytes. Sperm were motile within the spermatophore, but little or no evidence of motility was seen in the spermatheca or the vas deferens. Approximately 25% of sperm from the vas deferens became motile when dispersed in spermatic fluid plasma, the sperm‐bearing liquid of the vas deferens, indicating crowding to be a possible motion‐restraining factor. Fewer than 50% were motile in several saline media isosmotic with spermatic fluid plasma, whereas more than 90% became motile in distilled water or media at osmolalities near that of pond water. Motility in isosmotic solutions persisted beyond 12 hours, but at low osmolality ceased by 6 hours. When dispersed at higher osmolalities initial motility was low but increased to isosmotic levels by 12 hours. Responses to immersion in solutions of mannitol were similar to ones observed in saline solutions of equivalent osmolality. Dispersion in hydrogen ion concentrations between pH 4 and 9 did not affect the initial motility of sperm, but after 12 hours at pH 9, pH 4 or 5 movement was inhibited. In general, these data indicate a major role for osmolality in the enforced quiescence of sperm during storage and demonstrate that the low osmolality of pond water is primarily responsible for the activation of sperm in the spermatophore of the newt.