mRNA transport in yeast: time to reinvestigate the functions of the nucleolus.
mRNA transport in yeast: time to reinvestigate the functions of the nucleolus.
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酵母中的 mRNA 运输：是时候重新研究核仁的功能了。
DOI：
10.1091/mbc.6.4.357
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发表时间：
1995
影响因子：
3.3
通讯作者：
Tartakoff,AM
中科院分区：
文献类型：
--
作者：
Schneiter,R;Kadowaki,T;Tartakoff,AM
mRNA的核质转运对基因表达至关重要，可能是其调控的关键。遗传方法在酿酒酵母已经导致条件突变体在mRNA运输缺陷的鉴定。通过原位杂交检测到，大约24个基因的突变导致转录本的积累，这些转录本被困在细胞核的不同位置。对许多mRNA转运突变体的表型和分子分析表明，在酵母中，细胞核的功能不仅限于前核糖体的生物发生，而且可能对poly(A)+ RNA的转运也很重要。一些观察表明动物细胞核也具有类似的功能。
Nucleocytoplasmic transport of mRNA is vital to gene expression and may prove to be key to its regulation. Genetic approaches in Saccharomyces cerevisiae have led to the identification of conditional mutants defective in mRNA transport. Mutations in approximately two dozen genes result in accumulation of transcripts, trapped at various sites in the nucleus, as detected by in situ hybridization. Phenotypic and molecular analyses of many of these mRNA transport mutants suggest that, in yeast, the function of the nucleus is not limited to the biogenesis of pre-ribosomes but may also be important for transport of poly(A)+ RNA. A similar function of the animal cell nucleolus is suggested by several observations.