Stable Carbon Isotope Measurement of Individual Fatty Acids Using Gas Chromatography/Isotope Ratio Monitoring Mass Spectrometry
Stable Carbon Isotope Measurement of Individual Fatty Acids Using Gas Chromatography/Isotope Ratio Monitoring Mass Spectrometry
复制标题
使用气相色谱/同位素比监测质谱法测量单个脂肪酸的稳定碳同位素
DOI：
10.5702/massspec.42.315
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
R. Ishiwatari
中科院分区：
文献类型：
--
作者：
H. Naraoka;Keita Yamada;R. Ishiwatari
建立了一种气相色谱/同位素比值监测质谱（GC/IRMS）测定C16 ~ C30饱和正脂肪酸稳定碳同位素组成的方法。脂肪酸用BF 3/甲醇衍生成甲酯，三种正构烷烃用作内标。同位素质量平衡计算表明，酯化反应没有引起脂肪酸甲酯部分和所用甲醇之间的同位素分馏。因此，用甲醇的δ 13 C值对同位素进行了校正，增加一个碳原子。分析准确度（GC/IRMS测定的校正δ 13 C值与常规方法测定值的偏差）和精密度（标准偏差）对于90 - 360 ng的单个标准脂肪酸检测值均符合要求。多次分析平均准确度为±0.3‰，精密度在0.3‰以内。该方法用于海洋沉积物中C_（20）~ C_（30）正构脂肪酸的δ ~（13）C测定。
A gas chromatography/isotope ratio monitoring mass spectrometry (GC/IRMS) method was established for the determination of stable carbon isotopic compositions of individual saturated n-fatty acids ranging from C16 to C30. The fatty acids were derivatized to their methyl esters using BF3/methanol, and three n-alkanes were used as internal isotopic standard. Isotopic mass balance calculation indicated that the esterification caused no isotopic fractionation between the methyl ester moiety of fatty acid and methanol used. Therefore, isotopic correction was made by one additional carbon atom with the δ13C value of methanol. Analytical accuracy (deviation of the corrected δ13C values determined by GC/IRMS from those determined by a conventional method) and precision (standard deviation) were satisfactory for 90 to 360 ng of individual standard fatty acids examined. The accuracy was ±0.3‰ for average of several analyses, and the precision within 0.3‰. This method was applied to the δ13C determination of n-fatty acids ranging from C20 to C30 in a marine sediment.