ANALYSIS OF A 9.6 KB SEQUENCE FROM THE 3' END OF CANINE CORONAVIRUS GENOMIC RNA
ANALYSIS OF A 9.6 KB SEQUENCE FROM THE 3' END OF CANINE CORONAVIRUS GENOMIC RNA
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DOI：
10.1099/0022-1317-73-11-2849
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发表时间：
1992-11-01
影响因子：
3.8
通讯作者：
BROWN, TDK
中科院分区：
文献类型：
--
作者：
HORSBURGH, BC;BRIERLEY, I;BROWN, TDK
我们分析了犬冠状病毒（CCV）基因组RNA 3'端的结构，该病毒与传染性胃肠炎病毒（TGEV）、猪呼吸道冠状病毒（PRCV）和猫传染性腹膜炎病毒（FIPV）具有密切的抗原关系。从 CCV 菌株 Insavc-1 感染的 A72 细胞中分离的基因组 RNA 用于生成 cDNA 文库。鉴定出覆盖约 9.6 kb [从聚合酶基因 1b 的 3' 端到 Poly(A) 尾] 的重叠克隆。测序和随后的分析揭示了 10 个开放阅读框 (ORF)。其中三个编码主要冠状病毒结构多肽S、M和N；第四个编码小膜蛋白 SM，IBV 结构多肽 3c 的推定同源物，五个编码多肽，指定为 1b、3a、4、7a 和 7b，与 TGEV 或 FIPV 基因组中编码的推定非结构多肽同源。迄今尚未在该冠状病毒抗原组中鉴定出的额外 ORF 被指定为 3x。这些 ORF 与 TGEV、PRCV 和 FIPV 中的对应 ORF 的配对比对揭示了高水平的同一性，并强调了该组病毒成员之间的密切关系。
We have analysed the organization of the 3' end of the genomic RNA of canine coronavirus (CCV), a virus which has a close antigenic relationship to transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCV) and feline infectious peritonitis virus (FIPV). Genomic RNA isolated from CCV strain Insavc-1-infected A72 cells was used to generate a cDNA library. Overlapping clones, spanning approximately 9.6 kb [from the 3' end of the polymerase gene, 1b, to the poly(A) tail] were identified. Sequencing and subsequent analyses revealed 10 open reading frames (ORFs). Three of these code for the major coronavirus structural polypeptides S, M and N; a fourth codes for a small membrane protein, SM, a putative homologue of the IBV structural polypeptide 3c, and five code for polypeptides, designated 1b, 3a, 4, 7a and 7b, homologous to putative non-structural polypeptides encoded in the TGEV or FIPV genomes. An extra ORF which had not hitherto been identified in this antigenic group of coronaviruses was designated 3x. Pairwise alignment of these ORFs with their counterparts in TGEV, PRCV and FIPV revealed high levels of identity and highlighted the close relationship between the members of this group of viruses.