The Brassica mitochondrial plasmid can be sexually transmitted. Pollen transfer of a cytoplasmic genetic element
The Brassica mitochondrial plasmid can be sexually transmitted. Pollen transfer of a cytoplasmic genetic element
复制标题
芸苔属线粒体质粒可以通过性传播。
DOI：
10.1007/bf00332404
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
E. Swanson
中科院分区：
文献类型：
--
作者：
L. Erickson;R. Kemble;E. Swanson
11.3 kb线粒体（mt）质粒恢复到油菜（甘蓝型油菜）植物治愈的质粒（通过体外培养）杂交含质粒的男性，但不是通过嫁接无质粒芽到植物含有质粒。质粒恢复与线粒体DNA限制性图谱的改变无关，也不可能是从线粒体基因组中切除质粒序列的结果。花粉相关质粒的恢复可能是父本的细胞质通过花粉传递到雌性卵细胞的结果。在其他杂交组合中，无论是细胞质还是细胞核背景，质粒的花粉转移率平均为50%。这些实验表明，细胞质遗传元件可以是非母系遗传的，并表明与该元件相关的线粒体在受精过程中被传递到卵细胞。
The 11.3 kb mitochondrial (mt) plasmid was restored to rapeseed (Brassica napus) plants cured of the plasmid (by in vitro culture) by crossing to plasmid-containing males, but not by grafting plasmidless shoots onto plants containing the plasmid. Plasmid restoration is not associated with alterations in mt DNA restriction patterns nor is it likely the result of excision of plasmid sequences from the mt genome. Restoration of the mitochondrially-associated plasmid is probably the result of transmission of cytoplasm from the male parent through the pollen to the egg cell in the female. Pollen transfer of the plasmid also occurred in other crosses regardless of cytoplasmic or nuclear background and at an average rate of 50%. These experiments demonstrate that a cytoplasmic genetic element can be non-maternally inherited inBrassicaand suggest that the mitochondria with which this element is associated are transmitted to the egg cell during fertilization.