Carp (Cyprinus carpio) lipovitellin is a highly stable phospholipoglycoprotein with the same immunogenicity as vitellogenin
Carp (Cyprinus carpio) lipovitellin is a highly stable phospholipoglycoprotein with the same immunogenicity as vitellogenin
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鲤鱼脂卵黄蛋白是一种高度稳定的磷脂糖蛋白，与卵黄蛋白原具有相同的免疫原性
DOI：
10.1111/are.13587
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发表时间：
2018-04
影响因子：
2
通讯作者：
Shaoguo Ru
中科院分区：
文献类型：
--
作者：
Jun Wang;Zhenzhong Zhang;Yanyan Wei;Mingyi Zheng;Shaoguo Ru
Vitellogenin (Vtg) induction in carp (Cyprinus carpio) is a commonly used biomarker for oestrogenic contamination. However, the accurate quantification of Vtg was challenged because the easy degradation of Vtg standard would change the standard curves of the immunoassays. In this study, three yolk proteins were purified from carp ovarian extracts by a two‐step chromatographic method. The purified proteins were characterized as phospholipoglycoproteins with molecular weights of approximately 416, 398 and 383 kDa. In SDS‐PAGE, the purified proteins appeared as a major band of approximately 110 kDa and several faint bands. Based on these characteristics, purified proteins were identified as carp lipovitellin (Lv). Immunological analysis showed that anti‐Vtg antiserum reacted with the purified Lv. The results of stability analysis revealed that even heated at 60°C for 60 min, the electrophoretic patterns of carp Lv in native‐PAGE and SDS‐PAGE did not have a significant difference compared with the Lv solution stored at 4°C. Therefore, the purified carp Lv was confirmed to have similar immunogenicity with Vtg and possess exceptionally high stability, which would be helpful for the development of robust immunoassays for accurate quantification of carp Vtg.
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影响因子：
2.9
作者：
C. Tyler;J. Sumpter
通讯作者：
C. Tyler;J. Sumpter
DOI：
10.1016/s0305-0491(98)10125-6
发表时间：
1998-12
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
D. P. Lomax;William T. Roubal;James D Moore;Lyndal L. Johnson
通讯作者：
D. P. Lomax;William T. Roubal;James D Moore;Lyndal L. Johnson
影响因子：
--
作者：
T. Matsubara;K. Sawano
通讯作者：
T. Matsubara;K. Sawano
影响因子：
8.8
作者：
Bjorkblom, C.;Salste, L.;Kronberg, L.
通讯作者：
Kronberg, L.
DOI：
--
发表时间：
2015-06
期刊：
--
影响因子：
--
作者：
Oei;Io;Qs
通讯作者：
Oei;Io;Qs