Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome
Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome
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DOI：
10.1186/1476-511x-11-116
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发表时间：
2012-09-16
影响因子：
4.5
通讯作者：
Civeira, Fernando
中科院分区：
文献类型：
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作者：
Burillo, Elena;Mateo-Gallego, Rocio;Civeira, Fernando
背景资料：ω-3多不饱和脂肪酸（ω-3 PUFA）已被证明对预防心血管疾病有益，然而，它们发挥心血管保护作用的机制尚未阐明。有趣的是，其中一些保护作用也与HDL有关。本研究的假设是，ω-3 PUFA可以以甘油三酯非依赖性模式修饰HDL颗粒的蛋白质货物。该研究的目的是比较蛋白质组学的HDL之前和之后的omega-3 PUFAs补充diet.Methods：比较蛋白质组学分析6名吸烟者的主题HDL之前和之后的5周omega-3 PUFAs丰富的饮食已经执行。在改变的蛋白质中，发现丛生蛋白、对氧磷酶和apoAI增加，而纤连蛋白、α-1-抗胰蛋白酶、补充omega-3 PUFA后，补体C1 r亚组分和补体因子H降低。免疫检测试验证实了这些结果。上调的蛋白质与HDL的抗氧化、抗炎和抗动脉粥样硬化特性有关，而下调的蛋白质与调节补体激活和急性期反应有关。尽管研究中纳入的受试者人数很少，我们的研究结果表明，补充omega-3多不饱和脂肪酸可改变含载脂蛋白AI（LpAI）的脂蛋白，蛋白质组，并表明这些蛋白质的变化提高了颗粒的功能。
Background: Omega-3 poly-unsaturated fatty acids (omega-3 PUFAs) have demonstrated to be beneficial in the prevention of cardiovascular disease, however, the mechanisms by which they perform their cardiovascular protection have not been clarified. Intriguingly, some of these protective effects have also been linked to HDL. The hypothesis of this study was that omega-3 PUFAs could modify the protein cargo of HDL particle in a triglyceride non-dependent mode. The objective of the study was to compare the proteome of HDL before and after omega-3 PUFAs supplemented diet.Methods: A comparative proteomic analysis in 6 smoker subjects HDL before and after a 5 weeks omega-3 PUFAs enriched diet has been performed.Results: Among the altered proteins, clusterin, paraoxonase, and apoAI were found to increase, while fibronectin, alpha-1-antitrypsin, complement C1r subcomponent and complement factor H decreased after diet supplementation with omega-3 PUFAs. Immunodetection assays confirmed these results. The up-regulated proteins are related to antioxidant, anti-inflammatory and anti-atherosclerotic properties of HDL, while the down-regulated proteins are related to regulation of complement activation and acute phase response.Conclusions: Despite the low number of subjects included in the study, our findings demonstrate that omega-3 PUFAs supplementation modifies lipoprotein containing apoAI (LpAI) proteome and suggest that these protein changes improve the functionality of the particle.