The Arabidopsis peptide kiss of death is an inducer of programmed cell death
The Arabidopsis peptide kiss of death is an inducer of programmed cell death
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DOI：
10.1038/emboj.2011.14
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发表时间：
2011-03-16
期刊：
影响因子：
11.4
通讯作者：
Gallois, Patrick
中科院分区：
文献类型：
--
作者：
Blanvillain, Robert;Young, Bennett;Gallois, Patrick
程序性细胞死亡（PCD）在所有多细胞生物的防御和发育中起着关键作用。在植物中，我们对PCD各个阶段（尤其是早期阶段）所涉及的分子机制的了解存在很大差距。在这里，我们确定死亡之吻（KOD）编码一个25个氨基酸的肽，激活拟南芥中的PCD途径。两个突变等位基因的KOD表现出减少PCD的胚柄，一个单一的文件的细胞，支持胚胎发育，并减少PCD的根毛后，55 ℃的热休克。KOD表达被发现是由生物和非生物胁迫诱导的。此外，KOD的表达足以导致叶片或幼苗死亡，并激活半胱天冬酶样活性。此外，KOD诱导的PCD需要在叶片中的光，并抑制PCD抑制基因AtBax抑制剂1和p35。KOD表达导致线粒体膜的去极化，将KOD置于线粒体功能障碍之上，这是植物PCD的早期步骤。然而，KOD：：GFP融合定位于细胞的胞质溶胶中而不是线粒体中。The EMBO Journal（2011）30，1173-1183. doi：10.1038/daj.2011.14; 2011年2月15日在线发布
Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 degrees C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD::GFP fusion, however, localized in the cytosol of cells and not mitochondria. The EMBO Journal (2011) 30, 1173-1183. doi:10.1038/emboj.2011.14; Published online 15 February 2011