A solution-free crystal-mounting platform for native SAD
A solution-free crystal-mounting platform for native SAD
复制标题
适用于原生 SAD 的免解决方案晶体安装平台
DOI：
10.1107/s2059798320011584
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yao Min
中科院分区：
文献类型：
--
作者：
Yu Jian;Shinoda Akira;Kato Koji;Tanaka Isao;Yao Min
天然的SAD位相方法利用了大多数蛋白质中含有的S原子、核酸中的P原子或来自结晶溶液的其他轻原子的反常散射信号。这些信号非常微弱，需要仔细收集数据，这使得这种方法非常困难。增强异常信号的一种方法是使用长波X射线；然而，这些波长被路径中的材料吸收得更强。因此，开发了一种用于原生SAD数据采集的晶体安装平台，该平台可以去除晶体周围的溶液。该平台包括一个新的无溶液安装工具和一个自动机器人，它可以提取周围的溶液，闪冷晶体，并将环路插入Unipuck盒式磁带中，以用于同步加速器。用天然SAD方法从用该平台制备的晶体中成功地解决了8个蛋白质结构(包括2个新结构)。
The native SAD phasing method uses the anomalous scattering signals from the S atoms contained in most proteins, the P atoms in nucleic acids or other light atoms derived from the solution used for crystallization. These signals are very weak and careful data collection is required, which makes this method very difficult. One way to enhance the anomalous signal is to use long-wavelength X-rays; however, these wavelengths are more strongly absorbed by the materials in the pathway. Therefore, a crystal-mounting platform for native SAD data collection that removes solution around the crystals has been developed. This platform includes a novel solution-free mounting tool and an automatic robot, which extracts the surrounding solution, flash-cools the crystal and inserts the loop into a UniPuck cassette for use in the synchrotron. Eight protein structures (including two new structures) have been successfully solved by the native SAD method from crystals prepared using this platform.