TOF-SIMS analysis of exhaled particles from patients with asthma and healthy controls
TOF-SIMS analysis of exhaled particles from patients with asthma and healthy controls
复制标题
DOI：
10.1183/09031936.00195610
复制
发表时间：
2012-01-01
影响因子：
24.3
通讯作者：
Olin, A-C.
中科院分区：
文献类型：
--
作者：
Almstrand, A-C.;Josefson, M.;Olin, A-C.
呼出气中的颗粒物（PEx）可以反映呼吸道衬里液（RTLF）的组成;因此，有必要评估其作为呼吸道疾病生物标志物来源的潜力。在本研究中，我们使用飞行时间二次离子质谱（TOF-SIMS）和多变量分析比较了哮喘患者和对照组的PEx。15名经医生诊断为哮喘的非吸烟受试者和11名非吸烟健康对照者对仪器进行了10次连续的强制呼气。记录颗粒浓度，并通过TOF-SIMS分析收集在硅板上的颗粒样品。哮喘受试者呼出的颗粒数量显著低于对照组（p=0.03），哮喘受试者样品中不饱和磷脂与饱和磷脂的比例显著较低（0.25 vs 0.35; p=0.036）。正交偏最小二乘判别分析模型显示良好的分离之间的积极和消极的光谱。磷脂酰胆碱和磷脂酰甘油的分子离子和蛋白质片段被发现区分组。我们得出结论，分析PEx是一个有前途的方法来检查RTLF的组成。在目前的探索性研究中，我们可以区分哮喘和健康对照组的基础上TOF-SIMS光谱的PEX。
Particles in exhaled air (PEx) may reflect the composition of respiratory tract lining fluid (RTLF); thus, there is a need to assess their potential as sources of biomarkers for respiratory diseases. In the present study, we compared PEx from patients with asthma and controls using time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and multivariate analysis.Particles were collected using an instrument developed in-house. 15 nonsmoking subjects with physician-diagnosed asthma and 11 nonsmoking healthy controls performed 10 consecutive forced exhalations into the instrument. Particle concentrations were recorded and samples of particles collected on silicon plates were analysed by TOF-SIMS.Subjects with asthma exhaled significantly lower numbers of particles than controls (p=0.03) and the ratio of unsaturated to saturated phospholipids was significantly lower in samples from subjects with asthma (0.25 versus 0.35; p=0.036). Orthogonal partial least squares-discriminant analysis models showed good separation between both positive and negative spectra. Molecular ions from phosphatidylcholine and phosphatidylglycerol, and protein fragments were found to discriminate the groups.We conclude that analysis of PEx is a promising method to examine the composition of RTLF. In the present explorative study, we could discriminate between subjects with asthma and healthy controls based on TOF-SIMS spectra from PEx.