Influence of medium-chain fatty acids and short-chain organic acids on jejunal morphology and intra-epithelial immune cells in weaned piglets
Influence of medium-chain fatty acids and short-chain organic acids on jejunal morphology and intra-epithelial immune cells in weaned piglets
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DOI：
10.1111/jpn.12490
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发表时间：
2017-06-01
影响因子：
2.7
通讯作者：
Zentek, J.
中科院分区：
文献类型：
--
作者：
Ferrara, F.;Tedin, L.;Zentek, J.
中链脂肪酸（MCFA）和短链有机酸（SOA）是仔猪日粮中常用的饲料添加剂。在猪中描述MCFA或SOA对肠道形态和局部免疫系统影响的研究有限。本试验旨在研究日粮中添加SOA（0.41%富马酸和0.32%乳酸）或SOA与MCFA（0.15%辛酸和癸酸）的组合对断奶仔猪肠道形态和肠道免疫细胞的影响。将72头断奶仔猪随机分为3个试验组。每组6只动物的组织样本用于研究饲料添加剂对空肠绒毛长度和隐窝深度的潜在影响，并定量上皮内淋巴细胞（IEL）。通过免疫组织化学（IHC）和流式细胞术（FC）测定CD 3阳性IEL，而仅通过FC分析CD 2、CD 5、CD 8、CD 16和TCR阳性IEL。MCFA和SOA的补充没有显着影响形态学数据。结果表明，SOA能显著增加空肠上皮中CD 2（-）CD 8（-）T细胞的数量。猪空肠的IHC和FC分析均证实，大多数IEL表达表面标志物CD 3，可归类为细胞毒性T淋巴细胞。总之，数据表明SOA增加了空肠上皮中CD 2（-）CD 8（-）T细胞的比例。因此，SOA可能通过增加潜在效应细胞的组成数量来对局部免疫产生有益影响，以击败感染性疾病。
Medium-chain fatty acids (MCFA) and short-chain organic acids (SOA) are often used as feed additives in piglet diets. There are limited studies in pigs describing the impact of MCFA or SOA on gut morphology and the local immune system. The aim of this study was to investigate whether the supplementation of SOA (0.41% fumaric acid and 0.32% lactic acid), or the combination of SOA with MCFA (0.15% caprylic and capric acid) would have effects on gut morphology and intestinal immune cells in weaned piglets. A total number of 72 weaned piglets were randomly allocated into three experimental groups. Tissue samples of six animals per group were used to investigate the potential impact of the feed additives on villus length and crypt depth of the jejunum and to quantify intra-epithelial lymphocytes (IEL). CD3-positive IEL were determined via immunohistochemistry (IHC) and flow cytometry (FC), whereas CD2-, CD5-, CD8-, CD16- and TCR-positive IEL were only analysed by FC. The supplementation of MCFA and SOA did not significantly affect morphometric data. The FC data indicated that SOA significantly increased the quantity of CD2(-)CD8(-) T cells in the jejunum epithelium. Both IHC and FC analyses of pig jejunum confirmed that the majority of IEL expressed the surface marker CD3 and could be classified as cytotoxic T lymphocytes. In conclusion, the data indicated that SOA increased the proportion of CD2(-)CD8(-) T cells in the jejunal epithelium. Thus, SOA might enable a beneficial effect on the local immunity by increasing the constitutive number of potential effector cells to defeat infectious diseases.