Activity and activation of the granulocyte superoxide-generating system.
Activity and activation of the granulocyte superoxide-generating system.
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粒细胞超氧化物生成系统的活性和激活。
DOI：
10.1182/blood.v55.1.85.85
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发表时间：
1980
期刊：
影响因子：
20.3
通讯作者：
H. Cohen
中科院分区：
文献类型：
--
作者：
P. Newburger;M. Chovaniec;H. Cohen
吞噬细胞产生超氧化物（O2）作为吞噬作用的呼吸爆发的一部分。我们研究了人粒细胞#{176}2在体外响应佛波醇肉豆蔻酸酯乙酸酯（一种刺激O2生成的可溶性脂肪酸酯）的产生。该化合物的性质允许将O产生的连续测定适应于活性和活化的研究（即，从静止状态到活动状态的变化）。这两个过程，分别测量为O2产生的速率和其达到的滞后时间，对涉及温度的操纵有不同的响应。pH、佛波醇肉豆蔻酸酯乙酸盐浓度、N-乙基马来酰亚胺抑制。和能量代谢。pH值的变化深刻地影响速率，而不改变滞后时间。温度升高到3 ℃以上会降低速率，但会继续缩短滞后时间。因此。O2生成系统的活化和活性似乎具有不同的pH和温度依赖性。另外。对于佛波醇肉豆蔻酸酯乙酸酯刺激，它们具有不同的剂量-反应曲线：在高浓度下，速率达到平台，但滞后时间继续缩短。人类O2生成系统的活性对代谢抑制剂敏感。例如N-乙基马来酰亚胺。2-脱氧葡萄糖，以及2-脱氧葡萄糖与二硝基苯酚或氰化物的组合。活化速率（滞后时间）不受这些化合物的影响。这些发现表明，人类O2生成系统的活动及其激活是可分离的过程。此外。人外周血和豚鼠腹膜渗出物粒细胞的上述特征的比较揭示了几个重要的差异。
Phagocytic cells generate superoxide (02 ) as a part of the respiratory burst of phagocytosis. We studied human granulocyte #{176}2 production in vitro in response to phorbol myristate acetate, a soluble fatty acid ester that stimulates 02 generation. The properties of this compound permit the adaptation of a continuous assay of 0 production to the study of the activity and activation (i.e., the change from the resting to the active state) of the 02 -generating system. The two processes, measured respectively as the rate of 02 production and the lag time for its attainment, respond differently to manipulations involving temperature. pH, phorbol myristate acetate concentration, N-ethyl maleimide inhibition. and energy metabolism. Changes in pH profoundly influence the rate without changing the lag time. Increases in temperature above 3TC depress the rate but continue to shorten the lag time. Thus. the activation and activity of the 02 -generating system appear to have different pH and temperature dependency. In addition. they have different dose-response curves for phorbol myristate acetate stimulation: at high concentrations the rate reaches a plateau but the lag time continues to shorten. Activity of the human 02 -generating system is sensitive to metabolic inhibitors. such as N-ethyl maleimide. 2-deoxyglucose, and the combination of 2-deoxyglucose with dinitrophenol or cyanide. The rate of activation (lag time) is unaffected by these compounds. These findings show that the activity of the human 02 -generating system and its activation are separable processes. Furthermore. comparison of the above characteristics of human peripheral blood and guinea pig peritoneal exudate granulocytes reveals several important differences.